Project description:Exosomal long noncoding RNAs (lncRNAs) play an important role in the diagnosis and treatment of diseases. High-throughput sequencing was performed to determine the differential expression (DE) profiles of lncRNAs and mRNAs in plasma exosomes from patients with white matter hyperintensities (WMH) and healthy controls. Next, functional enrichment analysis and network interaction prediction were performed for DE RNAs, which were verified in a validation cohort (100 WMH and 100 healthy controls) using qRT–PCR. The diagnostic potential of candidate RNAs was evaluated by receiver operating characteristic (ROC) curves. The WMH group was then divided into subgroups according to the Fazekas scale and white matter lesion site, and the correlation of DE exo-lncRNAs in the subgroup was evaluated. We selected 6 DE RNAs (exo-lnc_011776, exo-lnc_011797, exo-lnc_004326, exo-lnc_013222, exo-WNK1 and exo-RPL18), and qRT–PCR confirmed that the expression trends of the differentially expressed genes (DEGs) was consistent with the RNA sequencing results. ROC curve analysis revealed that exo-lnc_011776, exo-lnc_011797, exo-lnc_004326, exo-WNK1 and exo-RPL18 exhibited diagnostic efficacy for WMH. Furthermore, exo-lnc_011797 was positively correlated with the severity of WMH and was significantly elevated in paraventricular matter hyperintensities patients. Our study found that five exosomal RNAs that have potential diagnostic value for WMH. Moreover, exo-lnc_011797 may be a predictor of the severity and location of WMH.
Project description:Tissue microRNAs (miRNAs) can detect cancers and predict prognosis. Several recent studies reported that tissue, plasma, and saliva miRNAs share similar expression profiles. In this study, we investigated the diagnostic value of salivary miRNAs (including whole saliva and saliva supernatant) for detection of esophageal cancer.
Project description:Tissue microRNAs (miRNAs) can detect cancers and predict prognosis. Several recent studies reported that tissue, plasma, and saliva miRNAs share similar expression profiles. In this study, we investigated the diagnostic value of salivary miRNAs (including whole saliva and saliva supernatant) for detection of esophageal cancer. By Agilent microarray, six deregulated miRNAs from whole saliva samples from seven patients with esophageal cancer and three healthy controls were selected. The six selected miRNAs were subjected to validation of their expression levels by RT-qPCR using both whole saliva and saliva supernatant samples from an independent set of 39 patients with esophageal cancer and 19 healthy controls.
Project description:Object: This study aims to analyze the differentially expressed circRNA in colon adenocarcinoma (COAD) and evaluate its diagnostic and prognostic value. Establish a new prog
Project description:In this study we aim to evaluate the diagnostic value of miRNAs for in the detection of acute myocardial infarction (AMI). We used microRNA microarray to screen differentially expressed microRNAs (miRNAs) in rat myocardial infarcted tissues compared with healthy control tissues. Then the expression of these miRNAs was further verified by quantitative real-time polymerase chain reaction (RT-PCR) in rat myocardial infarcted tissues and plasma. Finally, the diagnostic value of these circulating miRNAs was explored in 44 patients with AMI, 53 with coronary heart disease (CHD) and 56 healthy controls.