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Enterococcus faecalis V583

ABSTRACT: Enterococcus faecalis V583 Genome sequencing and assembly

PROVIDER: PRJNA82565 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
		Other
	SRR530528_1.fastq.gz	Fastqsanger.gz
	SRR530528_2.fastq.gz	Fastqsanger.gz
	SRR538800_1.fastq.gz	Fastqsanger.gz
	SRR538800_2.fastq.gz	Fastqsanger.gz

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Enterococcus Faecalis V583 secretome

Project description:Identification of proteins in the secretome of Enterococcus Faecalis V583 after growth on urine

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Comparative genomic hybridization of several Enterococcus faecalis strains compared to strain V583

Project description:Gene content in various Enterococcus faecalis strains compared to E. faecalis V583. Strains have been compared to the V583 strain by comparative genomic hybridization using genome-wide PCR-based microarrays representing the V583 genome. Genes have been deemed "present" or "divergent" in the various strains.

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Transcription profiling of Enterococcus faecalis V583 in response to NaCl

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The Enterococcus faecalis V583 transcriptional profile during survival within macrophages

Project description:Transcriptional profiling of E. faecalis V583 during intracellular survival compared to V583 grown to mid-log phase (OD 600 = 0.05) in THB + 1% glucose. In this study we report the complete intracellular E. faecalis V583 transcriptome following infection of RAW264.7 macrophages. During intracellular survival, approximately 45% of the V583 genome was differentially regulated including numerous genes involved in the oxidative stress, heat shock and SOS responses. We observed that the E. faecalis-containing phagosome was limited for glycolytic substrates, nucleotides, amino acids and numerous ions necessary for growth and protein function. Approximately 35% of the genes differentially regulated during survival within macrophages were of hypothetical/unknown function, suggesting that the V583 response to phagocytosis involves many previously unstudied loci. Here, we provide the first comprehensive study elucidating the transcriptional response of E. faecalis to phagocytosis, which may provide new targets for future studies.

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Project description:Grad-seq in Enterococcus faecalis V583 and Enterococcus faecium AUS004.

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Project description:Enterococcus faecalis V583 Raw sequence reads

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Transcriptome profiling of tdc cluster delection mutant of Enterococcus faecalis V583

Project description:The aim was to study the transcriptional profiling of the tdc cluster delection mutant E. faecalis V583 Δtdc (non-tyramine producer) compared to the wild type strain E. faecalis V583 (tyramine producer). We compared the expression profile of the strains grown in M17 medium with glucose as carbon source and suplemented with tyrosine.

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