Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Enterococcus faecalis V583 treated with SDS and bovine bile

ABSTRACT: The effects of bovine bile and SDS on transcriptional events were studied by means of genome wide microarrays in Enterococcus faecalis V583. Transcriptional profiles were obtained through time series experiments over periods of 120min for cells treated with bile and the combination of SDS and bile, and 360min. for SDS.

ORGANISM(S): Enterococcus faecalis

SUBMITTER: Margrete Solheim 

PROVIDER: E-TABM-242 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Transcriptional responses of Enterococcus faecalis V583 to bovine bile and sodium dodecyl sulfate.

Solheim Margrete M   Aakra Agot A   Vebø Heidi H   Snipen Lars L   Nes Ingolf F IF  

Applied and environmental microbiology 20070727 18

Resistance to bile is a prerequisite property of the gastrointestinal bacterial flora. Bile acids are powerful detergents, and resistance to sodium dodecyl sulfate (SDS) has therefore often been considered relevant to studies of bile resistance. We have studied the effects of bovine bile (BB) and SDS on Enterococcus faecalis V583 by traditional growth studies and microarrays. Transcriptional responses were studied by time course experiments. In the presence of BB (V583-BB) or SDS (V583-SDS), 308  ...[more]

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