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Cancer-Selective Mechanistic Targets of Diallyl Trisulfide (DATS) in Human Breast Cancer Cells Identified by RNA-seq Analysis

ABSTRACT: Cancer-Selective Mechanistic Targets of Diallyl Trisulfide (DATS) in Human Breast Cancer Cells Identified by RNA-seq Analysis

PROVIDER: PRJNA827109 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18769350_1.fastq.gz	Fastqsanger.gz
	SRR18769350_2.fastq.gz	Fastqsanger.gz
	SRR18769351_1.fastq.gz	Fastqsanger.gz
	SRR18769351_2.fastq.gz	Fastqsanger.gz
	SRR18769352_1.fastq.gz	Fastqsanger.gz

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Mechanistic Targets of Diallyl Trisulfide (DATS) in Human Breast Cancer Cells Identified by RNA-seq Analysis

Project description:Mechanistic Targets of Diallyl Trisulfide (DATS) in Human Breast Cancer Cells Identified by RNA-seq Analysis

| PRJNA726289 | ENA

Cancer-Selective Mechanistic Targets of Diallyl Trisulfide (DATS) in Human Breast Cancer Cells Identified by RNA-seq Analysis

Project description:We report differentially expressed genes by DATS exposure in MCF-10A human epithelial cell line and SK-BR-3 human breast cancer cell line

2022-04-20 | GSE200872 | GEO

Mechanistic Targets of Diallyl Trisulfide (DATS) in Human Breast Cancer Cells Identified by RNA-seq Analysis

Project description:We report differential gene expression changes by DATS in breast cancer cells

2021-04-30 | GSE173616 | GEO

Alteration of gene expression by diallyl trisulfide (DATS) administration in chemically-induced rat mammary tumors

Project description:We report altered gene expression profiles by DATS in chemically-induced rat mammary tumors

2021-12-03 | GSE189640 | GEO

Mechanistic Targets of Diallyl Trisulfide in Human Breast Cancer Cells Identified by RNA-seq Analysis.

Project description:Diallyl trisulfide (DATS), a metabolic by-product of processed garlic, is highly effective in inhibiting growth of human breast cancer cells in vitro and in vivo, but the underlying mechanisms are still not fully understood. In this study, we performed RNA-seq analyses using luminal-type (MCF-7) and basal-like (MDA-MB-231) human breast cancer cells to identify mechanistic targets of DATS. The Reactome Pathway Analysis revealed upregulation of genes associated with SLIT/ROBO tumor suppressor signaling following DATS treatment in both MCF-7 and MDA-MB-231 cells. However, the expression of SLIT2 and ROBO1 proteins or their downstream target C-X-C motif chemokine receptor 4 was not affected by DATS treatment in both cell lines. The Reactome as well as the Gene Ontology Pathways Analyses of the RNA-seq data from DATS-treated cells indicated downregulation of genes associated with G2/M phase cell cycle arrest in comparison with vehicle-treated control cells. Consistent with the RNA-seq data, DATS treatment caused a significant increase in the fraction of the G2/M population in both cell lines when compared to corresponding control cells. In addition, Ser10 phosphorylation of histone H3, a mitotic marker, was also increased significantly following DATS treatment in MCF-7 and MDA-MB-231 cells. These results indicate that while SLIT/ROBO signaling is not affected by DATS treatment, cell cycle arrest likely contributes to the antitumor effect of this phytochemical.

| S-EPMC8249207 | biostudies-literature

Alteration of gene expression by diallyl trisulfide (DATS) administration in chemically-induced rat mammary tumors

Project description:Alteration of gene expression by diallyl trisulfide (DATS) administration in chemically-induced rat mammary tumors

| PRJNA783932 | ENA

Next Generation Sequencing Facilitates Quantitative Analysis of control of Pseudomonas aeruginosa PAO1 and DATS-treated P. aeruginosa PAO1 Transcriptomes

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare transcriptome profiling of control of P. aeruginosa PAO1 (RNA-seq) to transcriptome profiling of DATS-treated P. aeruginosa PAO1 and to evaluate protocols for optimal high-throughput data analysis. Methods:LB medium (50 mL) was inoculated with exponential growth phase P. aeruginosa PAO1 at a concentration of 1.0E8 CFU/mL. Diallyl trisulfide was then added at a concentration of either 0 (control) or 0.63 μl/mL, respectively, in triplicate. All nine experiment groups were incubated in a water bath shaker at 37 ºC with a shaking rate of 180 rpm for 5 h. Cells were then sampled and centrifuged from the three control groups and three farnesol treatment groups, respectively. The cell precipitates were separately snap-frozen at -80ºC. Total RNA was isolated from cells using Trizol (Life Technologies, USA) according to the manufacturer’s protocol. Results: The transcriptome sequencing data of control and DATS-treated groups were compared and analyzed in the form of the following: control vs DATS. The details of the differentially expressed genes among the two groups showed that the amount of differential expressed genes between control group and DATS-treated group was high (total 5572, up-regulated 929, down-regulated 1432). Conclusions:

2021-12-31 | GSE178622 | GEO

Novel cancer-selective and disease subtype-independent mechanistic targets of withaferin A in human breast cancer cells

Project description:Novel cancer-selective and disease subtype-independent mechanistic targets of withaferin A in human breast cancer cells

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The sulfur stress response caused by sodium hydrosulfide, allicin and diallyl polysulfanes in Bacillus subtilis as revealed by transcriptomics

Project description:In this study, we compared the transcriptome signatures in response to sodium hydrosulfide (NaHS), allicin and diallyl tetrasulfane (DAS4) exposure in the Gram-positive bacterium Bacillus subtilis. This analysis revealed that NaHS, allicin and DAS4 caused a similar thiol-specific oxidative and electrophile stress response as well as protein and DNA damage.

2019-11-18 | GSE132981 | GEO

Mechanistic insights into anti-cancer properties of Curcumin and Andrographis in colorectal cancer

Project description:Mechanistic insights into anti-cancer properties of Curcumin and Andrographis in colorectal cancer

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