Project description:MicroRNAs (miRNAs) are involved in the regulation of important biological processes. Here, we describe a novel Drosophila miRNAs involved in ageing. We selected eight Drosophila miRNAs, displaying high homology with seed sequences of ageing-related miRNAs characterized in other species, and investigated whether the overexpression of these miRNAs affected ageing in Drosophila adult flies. The lifespan of adults overexpressing miR-305, a miRNA showing high homology with miR-239 in C. elegans, was significantly shorter. Conversely, a reduction in miR-305 expression led to a longer lifespan than that in control flies. miR-305 overexpression accelerated the impairment of locomotor activity, and promoted the age-dependent accumulation of poly-ubiquitinated protein aggregates in the muscle, as flies aged. Thus, we demonstrate that the ectopic expression of miR-305 has a deleterious effect on ageing in Drosophila. To identify the targets of miR-305, we performed RNA-Seq. We discovered several mRNAs encoding antimicrobial peptides and insulin-like peptides, whose expression changed in adults upon miR-305 overexpression. We further confirmed, by qRT-PCR, that miR-305 overexpression significantly decreases the mRNA levels of four antimicrobial peptides. As these mRNAs contain multiple sequences matching the seed sequence of miR-305, we speculate that a reduction in target mRNA levels, caused by ectopic miRNA expression, promotes ageing.

2018-02-14 | GSE107158 | GEO

Proteomic analysis of JF-305 derivative CPEB1 knockdown/overexpression cells

Project description:Pancreatic cancer is a leading cause of cancer death worldwide. Cytoplasmic polyadenylation element binding protein 1 (CPEB1) is a fundamental translation regulatory factor regulating the polyadenylation process of messenger RNA. However, its biological functions in pancreatic cancer are poorly understood. Here the CPEB1 gene was knocked down or overexpressed in JF-305 cell line. To investigate the role of CPEB1 and new interacting proteins, proteomic analysis was performed using LC-MS/MS approach.

2024-07-03 | PXD047241 | Pride

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