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Variant Polycomb complexes in Drosophila consistent with ancient functional diversity

ABSTRACT: Variant Polycomb complexes in Drosophila consistent with ancient functional diversity

PROVIDER: PRJNA833293 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18970063.fastq.gz	Fastqsanger.gz
	SRR18970064.fastq.gz	Fastqsanger.gz
	SRR18970065.fastq.gz	Fastqsanger.gz
	SRR18970066.fastq.gz	Fastqsanger.gz
	SRR18970067.fastq.gz	Fastqsanger.gz

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Variant Polycomb complexes in Drosophila consistent with ancient functional diversity

Project description:Polycomb group (PcG) mutants were first identified in Drosophila based on their failure to maintain proper Hox gene repression during development. The proteins encoded by the corresponding fly genes mainly assemble into one of two discrete Polycomb Repressive Complexes: PRC1 or PRC2. However, biochemical analyses in mammals have revealed alternative forms of PRC2, and multiple distinct types of non-canonical or variant PRC1. Through a series of proteomic analyses, we identify analogous PRC2 and variant PRC1 complexes in Drosophila, as well as a broader repertoire of interactions implicated in early development. Our data provide strong support for the ancient diversity of PcG complexes, and a framework for future analysis in a longstanding and versatile genetic system.

2022-04-29 | GSE201842 | GEO

Synergy between variant PRC1 complexes defines Polycomb-mediated gene repression (ChIP-Seq)

Project description:The Polycomb system modifies chromatin and plays an essential role in repressing gene expression to control normal mammalian development. However, the components and mechanisms that define how Polycomb protein complexes achieve this remain enigmatic. Here we use combinatorial genetic perturbation coupled with quantitative genomics to discover the central determinants of Polycomb-mediated gene repression in mouse embryonic stem cells. We demonstrate that canonical Polycomb repressive complex 1 (PRC1), which mediates higher order chromatin structures, contributes little to gene repression. Instead, we uncover an unexpectedly high degree of synergy between variant PRC1 complexes which is fundamental to gene repression. We further demonstrate that variant PRC1 complexes are responsible for distinct pools of H2A monoubiquitylation that are associated with repression of Polycomb target genes and silencing during X-chromosome inactivation. Together, these discoveries reveal a new variant PRC1-dependent logic for Polycomb-mediated gene repression.

2019-04-26 | GSE119618 | GEO

Synergy between variant PRC1 complexes defines Polycomb-mediated gene repression (RNA-Seq)

Project description:The Polycomb system modifies chromatin and plays an essential role in repressing gene expression to control normal mammalian development. However, the components and mechanisms that define how Polycomb protein complexes achieve this remain enigmatic. Here we use combinatorial genetic perturbation coupled with quantitative genomics to discover the central determinants of Polycomb-mediated gene repression in mouse embryonic stem cells. In contrast to prevailing views, we demonstrate that canonical Polycomb repressive complex 1 (PRC1), which mediates higher order chromatin structures, contributes little to gene repression. Instead, we uncover an unexpectedly high degree of synergy between variant PRC1 complexes which is fundamental to gene repression. We further demonstrate that variant PRC1 complexes are responsible for distinct pools of H2A monoubiquitylation that are associated with repression of Polycomb target genes and silencing during X-chromosome inactivation. Together, these discoveries reveal a new variant PRC1-dependent logic for Polycomb-mediated gene repression.

2019-04-26 | GSE119619 | GEO

Synergy between variant PRC1 complexes defines Polycomb-mediated gene repression

Project description:Synergy between variant PRC1 complexes defines Polycomb-mediated gene repression

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Variant Polycomb complexes in <i>Drosophila</i> consistent with ancient functional diversity.

Project description:Polycomb group (PcG) mutants were first identified in Drosophila on the basis of their failure to maintain proper Hox gene repression during development. The proteins encoded by the corresponding fly genes mainly assemble into one of two discrete Polycomb repressive complexes: PRC1 or PRC2. However, biochemical analyses in mammals have revealed alternative forms of PRC2 and multiple distinct types of noncanonical or variant PRC1. Through a series of proteomic analyses, we identify analogous PRC2 and variant PRC1 complexes in Drosophila, as well as a broader repertoire of interactions implicated in early development. Our data provide strong support for the ancient diversity of PcG complexes and a framework for future analysis in a longstanding and versatile genetic system.

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Drosophila variant Polycomb complex subunits LC-MS

Project description:Our proteomics anaylsis in Drosophila embryos revealed alternative forms of PRC2 and several distinct variant PRC1 types which are similar to polycomb complexes in mammals

2022-10-15 | PXD033615 | Pride

Synergy between variant PRC1 complexes defines Polycomb-mediated gene repression (ChIP-Seq)

Project description:Synergy between variant PRC1 complexes defines Polycomb-mediated gene repression (ChIP-Seq)

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Synergy between variant PRC1 complexes defines Polycomb-mediated gene repression (RNA-Seq)

Project description:Synergy between variant PRC1 complexes defines Polycomb-mediated gene repression (RNA-Seq)

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Polycomb complexes in skin epithelium development

Project description:Polycomb complexes in skin epithelium development

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Polycomb complexes in skin epithelium development III

Project description:Polycomb group (PcG) proteins are essential epigenetic transcriptional regulators. Traditionally, PcG proteins function as two multi-subunit complexes, Polycomb repressive complex 1 (PRC1) and PRC2, which largely overlap in their genomic binding and cooperate to establish repressive chromatin domains demarcated by H2AK119ub and H3K27me3. Here, using the developing skin epidermis as a paradigm, we uncovered a functional redundancy between Polycomb complexes in the repression of unwanted non-lineage genes.

2022-12-31 | GSE158323 | GEO

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