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Monocyte-derived alveolar macrophages autonomously determine severe outcome of respiratory viral infection [scRNA-seq]

ABSTRACT: Monocyte-derived alveolar macrophages autonomously determine severe outcome of respiratory viral infection [scRNA-seq]

PROVIDER: PRJNA834030 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19033251.fastq.gz	Fastqsanger.gz
	SRR19033252.fastq.gz	Fastqsanger.gz
	SRR19033253.fastq.gz	Fastqsanger.gz
	SRR19033254.fastq.gz	Fastqsanger.gz
	SRR19033255.fastq.gz	Fastqsanger.gz

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Monocyte-derived alveolar macrophages autonomously determine severe outcome of respiratory viral infection

Project description:Monocyte-derived alveolar macrophages autonomously determine severe outcome of respiratory viral infection

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Monocyte-derived alveolar macrophages autonomously determine severe outcome of respiratory viral infection [bulk RNA-seq]

Project description:Monocyte-derived alveolar macrophages autonomously determine severe outcome of respiratory viral infection [bulk RNA-seq]

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Monocyte-derived alveolar macrophages autonomously determine severe outcome of respiratory viral infection [scRNA-seq]

Project description:Various lung insults can result in replacement of resident alveolar macrophages (AM) by blood monocyte-derived (BMo)-AM. However, the dynamics of this process and its long-term consequences for respiratory viral infections remain unclear. Using several mouse models and a marker to unambiguously track fetal monocyte-derived (FeMo)-AM and BMo-AM, we established the kinetics and extent of replenishment and their function to recurrent influenza virus (IAV) infection. Massive loss of FeMo-AM resulted in rapid replenishment by self-renewal of survivors followed by generation of BMo-AM, which progressively outcompeted FeMo-AM over several months due to increased glycolytic and proliferative capacity. The presence of both naïve and experienced BMo-AM conferred severe pathology to IAV infection, which was associated with a pro-inflammatory phenotype. Furthermore, upon aging of naïve mice, FeMo-AM are gradually replaced by BMo-AM, which contribute to IAV disease severity in a cell autonomous manner. Taken together, our results suggest that origin rather than training of AM determines long-term function to respiratory viral infection and provide an explanation for increased severity in elderly.

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Monocyte-derived alveolar macrophages autonomously determine severe outcome of respiratory viral infection [bulk RNA-seq]

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Monocyte-derived alveolar macrophages autonomously determine severe outcome of respiratory viral infection

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-07-01 | GSE202002 | GEO

Analysis of tissue-derived alveolar and monocyte-derived alveolar macrophages at day 7 and day 14 post-nippostrongylus brasiliensis infection.

Project description:Alveolar macrophages from Cx3cR1-CreER x Rosa26-tandem dimer Tomato(TdT) control and infected mice were sort-purified on live CD45+CD64+F4/80+CD11c+SiglecF+ and then sorted based on expression of TdT, with tissue-resident cells being TdT- and monocyte-derived cells TdT+. Illumina-compatible libraries were generated using the NEBNext Ultra II DNA Library Prep Kit for Illumina (catalog no. E7645S; New England BioLabs) and sequenced using an Illumina NextSeq 550 in a single-end 75-base pair (bp) read configuration. Bulk RNA-seq analysis was performed in accordance with the nf-core RNA-seq guidelines v.1.4.2. The output reads were aligned to the GRCm38 genome using STAR, followed by gene count generation using featureCounts and StringTie. Read counts were normalized and compared for differential gene expression using DESeq2 with significance at a false discovery rate-adjusted P<0.05. We report that at day 7 monocyte-derived alveolar macrophages had a gene expression profile distinct from tissue-derived alveolar macrophages while by day 14 the monocyte-derived macrophages had assumed gene expression profiles more similar to tissue-derived alveolar macrophages, as determine by pairwise Euclidean distance calculation and principal components analysis.

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Transcription profiling of human peripheral blood monocyte (MONO), human peripheral blood monocyte derived macrophages treated with M-CSF (MACRO)and primary alveolar macrophages (BAL)

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