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RNA-seq in human keratinocytes HaCaT cell line

ABSTRACT: RNA-seq in human keratinocytes HaCaT cell line

PROVIDER: PRJNA836934 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19159464_1.fastq.gz	Fastqsanger.gz
	SRR19159464_2.fastq.gz	Fastqsanger.gz
	SRR19159465_1.fastq.gz	Fastqsanger.gz
	SRR19159465_2.fastq.gz	Fastqsanger.gz
	SRR19159466_1.fastq.gz	Fastqsanger.gz

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RNA-seq in human keratinocytes HaCaT cell line

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Project description:The keratinocyte cell line HaCaT was cultured for three days (proliferation) or for ten days (differentiation). RNA from cells at day3 was compared to RNA from cells at day10. The microarray hybridizations were performed in dye-swap procedure : RNA from cells at day3 was labeled with Cy3 (GSM4674, GSM4675, GSM4682, GSM4683) and then with cy5 (GSM4680, GSM4681). Keywords = cell density differentiation program in human keratinocytes

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Project description:The specific functional features of the epidermal keratinocytes are determined by the activity of many genes. The aim of the project was to characterize the role of HSPA2, a member of the HSPA chaperone family (HSP70), in human epidermal keratinocytes. The inactivation of the HSPA2 gene in the HaCaT line of spontaneously immortalized epidermal keratinocytes was performed by CRISPR/Cas9 gene editing system. Next, the effect of modifications on the transcriptomic profile of cells growing in a three-dimensional model of reconstructed human epidermis in vitro was investigated. The cells were grown at air-liquid interface culture on collagen-fibroblast matrix to achieve maximal level of HaCaT differentiation in RHE system.

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