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Human keratinocytes HaCaT LC-MS/MS

ABSTRACT: Triton X-100 has been used as a model substance to study the effects of irritants on the skin. HaCaT human keratinocyte cell line were treated with a non-cytotoxic dose of Triton X-100, as determined by the MTT assay and microscopically examination) for 48 h. The altered abundance of proteins from HaCaT keratinocytes exposed to Triton X-100 was analyzed by LC-MS/MS approach and quantified using Progenesis LC software.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Keratinocyte

SUBMITTER: Olesya Larina

LAB HEAD: Lisitsa AV

PROVIDER: PXD007776 | Pride | 2017-11-03

REPOSITORIES: Pride

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			Action	DRS
	170616ONA_07.mgf	Mgf
	170616ONA_07.raw	Raw
	170616ONA_07_160622035546.mgf	Mgf
	170616ONA_07_160622035546.raw	Raw
	170616ONA_07_160622052741.mgf	Mgf

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Publications

[Proteomic profiling of HaCaT keratinocytes exposed to skin damaging detergents].

Rusanov A L AL Petushkova N A NA Poverennaya E V EV Nakhod K V KV Larina O V OV Lisitsa A V AV Luzgina N G NG

Biomeditsinskaia khimiia 20171001 5

The effects of sodium dodecyl sulfate (25 mg/ml) and Triton X-100 (12.5 mg/ml and 25 mg/ml) on the HaCaT immortalized keratinocytes exposed to these surfactants for 48 h were studied. Using shotgun proteomics, a comparative analysis of the proteomic profiles of control and experimental cells after surfactants exposure was carried out. 260 common proteins were identified in control and experimental cells; 33 proteins were found in cells exposed to all three treatments, but not in control cells. T ...[more]

PMID: 29080872

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