Project description:To characterize the mitochondrial stress response induced by Doxycycline in vivo in liver and kidneys, we administered doxycycline (Dox) at 500 mg/kg/day (mpkd) in the drinking water to 9 weeks-old germ-free C57BL/6J mice for 16 days, hence eliminating the potential confounding impacts of Doxycycline on the microbiome.
Project description:To understand the effect of microbes on microRNAs in aorta, in this study, we examined expression of microRNA in the aorta of male (10 weeks old) germ-free mice and pathogen-free mice (control).
Project description:The goal of this study is to understand the effect of microbiota on the cell frequencies and gene expression in skin. To achieve that we performed single cell sequencing of bladder cells from germ-free (GF) and specific pathogen free (SPF) mice
Project description:The goal of this study is to understand the effect of microbiota on the cell frequencies and gene expression in skin. To achieve that we performed single cell sequencing of lung cells from germ-free (GF) and specific pathogen free (SPF) mice
Project description:The goal of this study is to understand the effect of microbiota on the cell frequencies and gene expression in liver. To achieve that we performed single cell sequencing of liver cells from germ-free (GF) and specific pathogen free (SPF) mice
Project description:The goal of this study is to understand the effect of microbiota on the cell frequencies and gene expression in the colon. To achieve that we performed single cell sequencing of colon epithelium and lamina propria cells from germ-free (GF) and specific pathogen free (SPF) mice
Project description:Epidermolysis bullosa simplex (EBS) is a skin disorder caused by mutations in keratin (K) 5 or K14 genes. Little effect therapy is available.Following clinical reports, we applied the small molecule doxycycline to K5-/- mice. We demonstrate that doxycycline extended the survival of neonatal K5-/- mice from less than 1 to up to 8 hours. Microarray and TaqMan real-time PCR showed a downregulation of MMP-13 and IL-1beta, indicating an effect of doxycycline on transcription. Our data offer a novel small molecule based therapy approach for EBS. Keywords: drug treatment, global gene expression comparative profile
Project description:The goal of this study is to understand the effect of microbiota on the cell frequencies and gene expression in adipose tissue. To achieve that we performed single cell sequencing of epididymal fat pad cells from germ-free (GF) and specific pathogen free (SPF) mice
Project description:The goal of this study is to understand the effect of microbiota on the cell frequencies and gene expression in the small intestine . To achieve that we performed single cell sequencing of small intestine epithelium and lamina propria cells from germ-free (GF) and specific pathogen free (SPF) mice