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Effect of intervention with AAV8-scFv-E06 on FPC diet induced transcriptomic chnages in whole liver tissue

ABSTRACT: Effect of intervention with AAV8-scFv-E06 on FPC diet induced transcriptomic chnages in whole liver tissue

PROVIDER: PRJNA837959 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19201046_1.fastq.gz	Fastqsanger.gz
	SRR19201046_2.fastq.gz	Fastqsanger.gz
	SRR19201047_1.fastq.gz	Fastqsanger.gz
	SRR19201047_2.fastq.gz	Fastqsanger.gz
	SRR19201048_1.fastq.gz	Fastqsanger.gz

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Effect of intervention with AAV8-scFv-E06 on FPC diet induced transcriptomic chnages in whole liver tissue

Project description:To investigate genes regulated by expression scFv-E06 in the liver of mice fed FPC diet

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RNA-seq of rd1 retinal microglia after treatment with AAV8-GFP vs. AAV8-GFP + AAV8-TGFB1

Project description:RNA-seq of rd1 retinal microglia after treatment with AAV8-GFP vs. AAV8-GFP + AAV8-TGFB1

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RNA-seq of rd1 retinal microglia after treatment with AAV8-GFP vs. AAV8-GFP + AAV8-TGFB1

Project description:Transcriptional profiling of microglia (seven biological replicates per experimental condition) or non-microglia (four biological replicates total). One thousand microglia (CD11b+ Ly6G/Ly6C-) or non-microglia cells (CD11b-) from P30 rd1 (FVB) retinas infected with either AAV8-GFP or AAV8-GFP + AAV8-TGFB1 were sorted into 10 µL of Buffer TCL (Qiagen) containing 1% beta-mercaptoethanol and immediately frozen on dry ice. Samples were subsequently sent to the Broad Institute Genomics Platform for cDNA library synthesis and sequencing using a modified Smart-Seq2 protocol with an expected coverage of ~6 million reads per sample. Prior to analysis, reads were subjected to quality control measures and mapped to the GRCm38.p6 reference genome. Reads assigned to each gene were then quantified using featureCounts and normalized and analyzed for differential expression using DESeq2.

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Analysis of Escherichia coli CyDisCo proteome changes in response to the expression of scFv and the misfolded scFv

Project description:E. coli CyDisCo strain enables a high yield secretion of disulfide bond-containing proteins to the periplasm via Twin-arginine (Tat) pathway. Introducing two exogenous oxidases: the yeast sulfydryl oxidase (Erv1p) and human protein disulfide isomerase (PDI), the CyDisCo strain changes the cytoplasm into an oxidized environment, where the disulfide bonds can efficiently be formed. In this study, we analyzed the proteome changes upon the expression of disulfide bond-containing scFv and the misfolded scFv in the CyDisCo strain. The correctly folded protein is secreted to the periplasm, while the misfolded protein accumulates exclusively in the inclusion body fraction. We observed a high number of significant changes mostly in proteins associated with protein folding and degradation, oxidative stress, membrane transport and integrity.

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Transcription profiling by array of liver from C57BL6 mice transfected with AAV8 vectors expressing either GFP or human macrophage inhibitory factor (MIF) or none (PBS).

Project description:MIF is a general mediator of inflammatory responses and is associated with several inflammatory diseases including atherosclerosis. Therefore MIF is currently investigated as a therapeutic target for atherosclerosis. Generation of AAV8 vectors expressing human MIF was initiated to overexpress human MIF in AAV8 target tissues (liver, heart, muscles) of C57/Bl6 to study biological function of MIF in vivo and to provide an animal model for testing of MIF antibodies. Aim of the gene expression profiling experiment: Livers of AAV8-CMV-MIF treated animals in order to identify established and potential novel target genes of MIF induced signalling

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Herbaspirillum sp. JGI 000192CP-E06

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Herbaspirillum sp. JGI 000192CP-E06

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