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Integrated mapping of the dog epigenome (MBD-seq)

ABSTRACT: Integrated mapping of the dog epigenome (MBD-seq)

PROVIDER: PRJNA838585 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19224300_1.fastq.gz	Fastqsanger.gz
	SRR19224300_2.fastq.gz	Fastqsanger.gz
	SRR19224301_1.fastq.gz	Fastqsanger.gz
	SRR19224301_2.fastq.gz	Fastqsanger.gz
	SRR19224302_1.fastq.gz	Fastqsanger.gz

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Integrated mapping of the dog epigenome

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Project description:Dogs have become a valuable model in exploring multifaceted diseases and biology relevant to human health. Despite large-scale dog genome projects producing high-quality draft references, a comprehensive annotation of functional elements is still lacking. We addressed this through integrative next-generation sequencing of transcriptomes paired with five histone marks and DNA methylome profiling across 11 tissue types, deciphering the dog’s epigenetic code by defining distinct chromatin states, super-enhancer and methylome landscapes, and thus showed that these regions are associated with a wide range of biological functions and cell/tissue identity. In addition, we confirmed that the phenotype-associated variants are enriched in tissue-specific regulatory regions and, therefore, the tissue of origin of the variants can be traced. Ultimately, we delineated conserved and dynamic epigenomic changes at the tissue- and species-specific resolutions. Our study provides an epigenomic blueprint of the dog that can be used for comparative biology and medical research.

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