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Genome-wide maps of m6a state in RA FLSs with or without ALKBH5 knockdown.

ABSTRACT: Genome-wide maps of m6a state in RA FLSs with or without ALKBH5 knockdown.

PROVIDER: PRJNA839340 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19265067_1.fastq.gz	Fastqsanger.gz
	SRR19265067_2.fastq.gz	Fastqsanger.gz
	SRR19265068_1.fastq.gz	Fastqsanger.gz
	SRR19265068_2.fastq.gz	Fastqsanger.gz
	SRR19265069_1.fastq.gz	Fastqsanger.gz

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Genome-wide maps of m6a state in RA FLSs with or without ALKBH5 knockdown.

Project description:To investigate how ALKBH5 modulates RA FLSs functions, we conducted m6A sequencing (MeRIP-seq) of RA FLSs. Consistent with previous studies, in both scramble controls and ALKBH5 knockdown cells, m6A modifications were highly enriched within the GGAC motif. m6A peaks are located mainly in the protein-coding region (CDS) and 3’ untranslated region (3’ UTR) of mRNA transcripts

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Gene expression profile of RA FLSs with or without ALKBH5 knockdown

Project description:To investigate how ALKBH5 modulates RA FLSs functions, we conducted RNA sequencing of RA FLSs. The data were analyzed for differential expression with a nominal P<0.05. Through analysis of the input RNA-seq data, we identified significantly expressed 1956 genes(FC≥1.2).

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Gene expression profile of RA FLSs with or without ALKBH5 knockdown

Project description:Gene expression profile of RA FLSs with or without ALKBH5 knockdown

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ALKBH5 is a New Mammalian RNA Demethylase

Project description:N6-methyladenosine (m6A) is the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Here we report ALKBH5 as a new mammalian demethylase that oxidatively removes the m6A modification in mRNA in vitro and inside cells. This demethylation activity of ALKBH5 significantly affects mRNA export and RNA metabolism as well as the assembly of mRNA processing factors in nuclear speckles. Alkbh5-deficient male mice are characterized by impaired fertility resulting from apoptosis that affects meiotic metaphase-stage spermatocytes. In accordance with this defect, we have identified in mouse testes 1552 differentially expressed genes which cover broad functional categories and include spermatogenesis-related mRNAs involved in the p53 functional interaction network. We show that Alkbh5-deficiency impacts the expression levels of some of these mRNAs, supporting the observed phenotype. The discovery of this new RNA demethylase strongly suggests that the reversible m6A modification plays fundamental and broad functions in mammalian cells. RNA-seq in two cell types

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Chromatin state maintains human myeloid leukemia by regulating m6A demethylase ALKBH5 expression [RNA-Seq]

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