Project description:ACTL6A has been identified as a transcriptional regulator and driving pathways that are of specific benefit to the malignant elements within the tumor. Here, we aimed to find genes that are regulated by ACTL6A by CUT&Tag. BRG1 is the catalytic subunit of the SWI/SNF chromatin-remodeling complex. Nuclear factor (erythroid-derived 2)-like 2 (NFE2L2, NRF2) is a transcription factor that governs the antioxidant pathway. IgG was used as a control.

2023-04-20 | GSE216350 | GEO

ACTL6a Enforces the Epidermal Progenitor State by Suppressing SWI/SNF-Dependent Induction of KLF4

Project description:Somatic progenitors suppress differentiation to maintain tissue self-renewal. While epigenetic regulators of DNA and histone modifications can support such repression, a role for nuclear actin-like proteins is unclear. In epidermis, ACTL6a/BAF53A was found enriched in progenitors and down-regulated during differentiation. Conditional ACTL6a deletion abolished epidermal self-renewal and induced terminal differentiation, whereas ectopically expressed ACTL6a suppressed differentiation. Among known activators of epidermal differentiation, KLF4 was found to control 227 genes also regulated by ACTL6a. ACTL6a loss upregulated KLF4 and its target genes, effects that were blocked by KLF4 depletion. Among multiple ACTL6a-interacting epigenetic regulators, the SWI/SNF complex was required for KLF4 activation and differentiation. In progenitors, ACTL6a loss led to enhanced SWI/SNF binding to the promoters of KLF4 and other differentiation genes. ACTL6a thus maintains the undifferentiated progenitor state, in part by suppressing SWI/SNF complex-enabled induction of KLF4. Gene expression analysis: To identify the gene set controlled by ACTL6a in human keratinocyte. Total RNA was isolated in biologic duplicate from cells with ACTL6a loss as compared to controls, and hybridized to Affymetrix HG-U133 2.0 Plus arrays.

2012-11-30 | E-GEOD-36222 | biostudies-arrayexpress

Expression data from gastric cancer cell line HGC27 after knockdown of SNAIL

Project description:Expression data from gastric cancer cell line HGC27 after knockdown of SNAIL

| PRJNA608543 | ENA

ACTL6a Enforces the Epidermal Progenitor State by Suppressing SWI/SNF-Dependent Induction of KLF4

2012-11-30 | GSE36222 | GEO

Expression data from endometrial adenocarcinoma cell line ISK with and without EZH2 knockdown

Project description:Expression data from endometrial adenocarcinoma cell line ISK with and without EZH2 knockdown

| PRJNA578891 | ENA

Expression data from Knockdown of ACTL6A in SNU638 cell line

Project description:Gastric cancer malignant progression depends on the aberrant oncogenic expressions. During tumorigenesis, the oncogeneic genes mutation or expression, following specific sets of genes are up-regulated or down-regulated. Some of them, for instance, control metabolism reprogramming to promote tumor growth and maliganant progression.

2023-04-20 | GSE203657 | GEO

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