Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome-wide gene expression Haloferax volcanii under oxidative stress

ABSTRACT: Genome-wide gene expression Haloferax volcanii under oxidative stress

PROVIDER: PRJNA842435 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR19410007_1.fastq.gz	Fastqsanger.gz
	SRR19410007_2.fastq.gz	Fastqsanger.gz
	SRR19410008_1.fastq.gz	Fastqsanger.gz
	SRR19410008_2.fastq.gz	Fastqsanger.gz
	SRR19410009_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 60

Similar Datasets

Genome-wide gene expression Haloferax volcanii under oxidative stress

Project description:The goals of this study are to compare NGS-derived transcriptome profiling (RNA-seq) of H. volcanii upon treatment of NaOCl.

2022-05-25 | GSE204840 | GEO

Transcriptional Landscape and Regulatory Roles of Small Noncoding RNAs in the Oxidative Stress Response of the Haloarchaeon Haloferax volcanii

Project description:We demonstrate here the transcriptional landscape and functional roles of sRNAs specifically in the regulation of the oxidative stress response of the model haloarchaeon Haloferax volcanii. We sequenced 5 biological replicates of H. volcanii strain H53 under no challenge and oxidative stress (H2O2) conditions at mid-exponential phase (OD 0.4). Thousands of sRNAs, both intergenic and antisense, were discovered using strand-specific sRNA-seq, comprising around 30% of the transcriptome during non-challenged and oxidative stress conditions. Antisense sRNAs were found to overlap both 5’ and 3’ UTRs of mRNAs revealing a hybrid system between Eukarya (3’ UTR) and Bacteria (5’ UTR) sRNA-silencing systems, as well as targeting the coding sequence (CDS) of mRNAs, a unique system to Archaea. We identified hundreds of differentially expressed sRNAs in response to hydrogen peroxide induced oxidative stress in H. volcanii. A majority of these sRNAs are lowly expressed compared to mRNAs. The sRNAs could be classified in two populations based on expression patterns: those that are up-regulated and those that are down-regulated during oxidative stress. Targets of antisense sRNAs decreased in expression when sRNAs were up-regulated indicating that sRNAs are likely playing a negative regulatory role on mRNA targets at the transcript level. Target enrichment of sRNAs included mRNAs involved in transposons, chemotaxis signaling, peptidase activity, transcription factors, and secondary metabolite regulation.

2018-02-20 | GSE103893 | GEO

Haloferax volcanii DS2

Project description:Haloferax volcanii xylose

| PRJNA953037 | ENA

Haloferax volcanii DS2

Project description:Haloferax volcanii IPTG

| PRJNA953035 | ENA

Haloferax volcanii DS2

Project description:Haloferax volcanii Arabinose

| PRJNA953033 | ENA

Haloferax volcanii DS2

Project description:Haloferax volcanii Maltose

| PRJNA953034 | ENA

Response of Haloferax volcanii to low-iron stress

Project description:We analyzed the response of the haloarchaeal model organism Haloferax volcanii to low iron stress on the proteome level by label-free mass spectrometry. Cells grown in minimal medium with normal iron levels were compared to those grown under low iron conditions. We separated the samples into soluble and membrane fraction to focus specifically on import/export processes which are frequently associated with metal uptake. Using a comprehensive annotated spectral library and data-independent acquisition mass spectrometry, we identified significant changes to both the membrane and the soluble proteomes of Haloferax volcanii under iron starvation.

2024-08-04 | PXD029036 | Pride

Haloferax volcanii DS2

Project description:Haloferax volcanii Mating Transcriptome

| PRJNA632928 | ENA

Haloferax volcanii DS2

Project description:Haloferax volcanii Nucleosome map

| PRJNA174818 | ENA

Haloferax volcanii

Project description:Analysis of the Haloferax volcanii Transcriptome

| PRJNA119335 | ENA