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Epigenetic analysis of hepatocyte-like cells from MSC-Ucs.

ABSTRACT: Epigenetic analysis of hepatocyte-like cells from MSC-Ucs.

PROVIDER: PRJNA846304 | ENA |

REPOSITORIES: ENA

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Epigenetic analysis of hepatocyte-like cells from MSC-Ucs.

Project description:Global DNA methylation profiling of hepatocyte-like cells. We established a protein-based artificial transcription system and successfully engineered human hepatocyte-like cells from human umbilical cord-derived mesenchymal stem cells (MSC-UCs). We utilized four artificial transcription factors (ATFs) that targeted hepatocyte nuclear factor (HNF)1α, HNF3γ, HNF4α and GATA4 (4F), and found that treatment with 4F for 3 (4Fd3) or 5 (4Fd5) days converted MSC-UCs to hepatocyte-like cells. We then examined global DNA methylation profiles of 4Fd3, 4Fd5, NTP-EGFP protein treated cells (NE) and non-treated cells (NC).

2022-06-09 | GSE205546 | GEO

Epigenetic profiling of hepatocyte, reprogrammed hepatocyte, and biliary cells

Project description:Epigenetic profiling of hepatocyte, reprogrammed hepatocyte, and biliary cells

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Epigenetic profiling of hepatocyte, reprogrammed hepatocyte, and biliary cells

Project description:ATAC-seq analysis of mouse hepatocyte, reprogrammed hepatocyte, and biliary cells

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Gene expression profiling of hepatocyte-like cells from human mesenchymal stem cells

Project description:We established a protein-based artificial transcription system and successfully engineered human hepatocyte-like cells from human umbilical cord-derived mesenchymal stem cells (MSC-UCs). We utilized four artificial transcription factors (ATFs) that targeted hepatocyte nuclear factor (HNF)1α, HNF3γ, HNF4α and GATA4 (4F), and found that treatment with 4F for 5 days converted MSC-UCs to hepatocyte-like cells (4F-Heps). We then examined gene expression profiles of 4F-Heps and compared them with those of non-treated MSC-UCs (NC) or MSC-UCs treated with NTP-EGFP protein as control protein (NE).

2022-06-09 | GSE205543 | GEO

Expression analysis of Hepatocyte like-cells derived SHEDs (SHED-Hep cells)

Project description:Comparison of whole genome gene expression profiles of stem cells from exfoliated decidous teeth (SHEDs) ,Hepatocyte like-cells derived SHEDs (SHED-Hep cells), and human primary hepatocytes. We showed that the profile of three different donors of MSC-Hep cells was close to that of human hepatocytes, but separate from that of three different donors of undifferentiated MSCs

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Promoter Capture-C analysis of Hepatocyte-like cells

Project description:Promoter Capture-C analysis of Hepatocyte-like cells

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HBO1 is an epigenetic determinant of hepatocyte-to-ductal reprogramming

Project description:Spontaneous cellular reprogramming is rare, but has been observed in adult cells. This is most evident in the mammalian liver, where hepatocytes undergo physiological reprogramming to generate functional biliary epithelial cells (BECs) in response to injury. The underlying mechanisms driving this cell fate switch remain unclear, however. Here, we characterize epigenetic changes occurring during this transition at the single cell level, and show that reprogramming occurs synchronously and deterministically, though reprogrammed cells retain epigenetic hepatocyte memory. An in vivo CRISPR screen reveals the histone acetyltransferase-HBO1 functions as a critical barrier to hepatocyte reprogramming via acetylation of H3K14. HBO1 depletion accelerates BEC-specific chromatin remodeling and allows for the full resolution of the hepatocyte chromatin landscape. Mechanistically, HBO1 is recruited by the YAP to TEAD target sites to negatively its modulate chromatin-accessibility, DNA-binding, and transcriptional-output, thus acting as an epigenetic brake for YAP/TEAD function. Our work here delineates epigenetic trajectories of a physiological reprogramming process and identifies HBO1 as potential target for hepatocyte trans-differentiation therapeutic strategies.

2025-05-20 | GSE168828 | GEO

Quantitative proteomic characterization of lung-MSC and bone marrow-MSC using DIA-mass spectrometry

Project description:Mesenchymal stromal cells (MSC) are ideal candidates for cell therapies, due to their immune-regulatory and regenerative properties. We have previously reported that lung-derived MSC are tissue-resident cells with lung-specific properties compared to bone marrow-derived MSC. Assessing relevant molecular differences between lung-MSC and bone marrow-MSC is important, given that such differences may impact their behavior and potential therapeutic use. Here, we present an in-depth mass spectrometry (MS) based strategy to investigate the proteomes of lung-MSC and bone marrow-MSC. The MS-strategy relies on label free quantitative data-independent acquisition (DIA) analysis and targeted data analysis using a MSC specific spectral library. We identified several significantly differentially expressed proteins between lung-MSC and bone marrow-MSC within the cell layer (352 proteins) and in the conditioned medium (49 proteins). Bioinformatics analysis revealed differences in regulation of cell proliferation, which was functionally confirmed by decreasing proliferation rate through Cytochrome P450 stimulation. Our study reveals important tissue-specific differences within proteome and matrisome profiles between lung- and bone marrow-derived MSC that may influence their behavior and affect the clinical outcome when used for cell-therapy.

2018-10-18 | PXD006419 | Pride

Transcriptome analysis of human mononuclear blood cells post UC-MSC treatment

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Genome-wide analysis of human bone marrow-derived MSC cells in comparision with MSC with EGR1 knockdown or EGR1 overexpressing cells

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