Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Transcriptomes of undifferentiated normal human urothelium

PROVIDER: PRJNA847878 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR19614429_1.fastq.gz	Fastqsanger.gz
	SRR19614429_2.fastq.gz	Fastqsanger.gz
	SRR19614430_1.fastq.gz	Fastqsanger.gz
	SRR19614430_2.fastq.gz	Fastqsanger.gz
	SRR19614431_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

Transcriptomes of in vitro differentiated normal human urothelium

Project description:Transcriptomes of in vitro differentiated normal human urothelium

| PRJNA610264 | ENA

Hypermethylation of CpG Islands and Shores adjacent to mirtron and microRNA genes in bladder cancer

Project description:We examined mirtron and miR expression in bladder cancer cell line following DNA Methyltransferase inhibition and normal urothelium using human Agilent microRNA microarrays.

2011-02-17 | GSE22380 | GEO

Hypermethylation of CpG Islands and Shores adjacent to mirtron and microRNA genes in bladder cancer

Project description:We examined mirtron and miR expression in bladder cancer cell line following DNA Methyltransferase inhibition and normal urothelium using human Agilent microRNA microarrays. Bladder cancer cell lines (EJ, RT4 and RT112) were treated with 5-azacytidine ''Aza'' (2uM) for 5-7 days and normal urothelium (NHU) were subjected for microRNA microarray analysis. Analysis were performed in triplicates or more.

2011-02-17 | E-GEOD-22380 | biostudies-arrayexpress

Kruppel-like factor 5 is Required for Urothelial Maturation

Project description:Kruppel-like transcription factor 5 (Klf5) is expressed during late embryogenesis in the forming murine bladder urothelium. Targeted disruption of the Klf5flox alleles by the ShhGfpCre transgene resulted in failure of the bladder urothelium to mature accompanied by hydronephrosis, hydroureter, and vesicoureteric reflux in all E18.5 fetuses. The bladder urothelium did not stratify nor did it express terminal differentiation markers characteristic of basal, intermediate, and umbrella cells including keratins 20, 14, and 5, and uroplakins. At E18.5, an ectopic alpha smooth muscle actin positive layer of cells was identified subjacent to the undifferentiated Klf5-deficient urothelium. The effects of Klf5 deficiency were unique to the urothelium since maturation of the epithelium comprising the bladder neck and urethra were unaffected by the lack of KLF5. mRNA microarray analysis of whole E14.5 control and Klf5 deficient bladders identified Ppar-gamma and Grhl3 as putative downstream intermediary transcription factors that regulate urothelial maturation. Transient transfection assays demonstrated that KLF5 regulated expression of the mGrhl3 promoter. These observations show that alterations in maturation of the bladder urothelium alone are sufficient to induce bladder dysfunction leading to prenatal hydronephrosis.

2011-08-02 | GSE27014 | GEO

Kruppel-like factor 5 is Required for Urothelial Maturation

Project description:Kruppel-like transcription factor 5 (Klf5) is expressed during late embryogenesis in the forming murine bladder urothelium. Targeted disruption of the Klf5flox alleles by the ShhGfpCre transgene resulted in failure of the bladder urothelium to mature accompanied by hydronephrosis, hydroureter, and vesicoureteric reflux in all E18.5 fetuses. The bladder urothelium did not stratify nor did it express terminal differentiation markers characteristic of basal, intermediate, and umbrella cells including keratins 20, 14, and 5, and uroplakins. At E18.5, an ectopic alpha smooth muscle actin positive layer of cells was identified subjacent to the undifferentiated Klf5-deficient urothelium. The effects of Klf5 deficiency were unique to the urothelium since maturation of the epithelium comprising the bladder neck and urethra were unaffected by the lack of KLF5. mRNA microarray analysis of whole E14.5 control and Klf5 deficient bladders identified Ppar? and Grhl3 as putative downstream intermediary transcription factors that regulate urothelial maturation. Transient transfection assays demonstrated that KLF5 regulated expression of the mGrhl3 promoter. These observations show that alterations in maturation of the bladder urothelium alone are sufficient to induce bladder dysfunction leading to prenatal hydronephrosis. Total RNA from E14.5 whole bladders isolated from Klf5D/D and Klf5+/+ShhGfpCre+ embryos (n=3 samples/genotype, each sample a pool of two bladders) was isolated using the Qiagen MicroRNA Kit. The cDNA was then hybridized according to the manufacturer’s protocol to the Affymetrix Mouse Gene 1.0 ST Array

2011-08-01 | E-GEOD-27014 | biostudies-arrayexpress

Deciphering epigenetic and transcriptional alterations after Kmt2c/2d knock-out in mouse bladder urothelial cells

Project description:KMT2C and KMT2D are two of the most frequently mutated genes in histologically normal human bladder urothelium. In this study, we sought to model Kmt2c/d LOF mutations and investigate the biological mechanism of Kmt2c/d LOF mutations in bladder cancer development.

2024-08-09 | GSE180947 | GEO

Rat-bladder-OAB - Proteomic analysis of urothelium of rats with detrusor overactivity induced by bladder outlet obstruction

Project description:Overactive bladder (OAB) syndrome is a condition that has four symptoms: urgency, urinary frequency, nocturia, and urge incontinence and negatively affects a patient’s life. Recently, it is considered that the urinary bladder urothelium is closely linked to pathogenesis of OAB. However, the mechanisms of pathogenesis of OAB at the molecular level remain poorly understood, mainly as a result of lack of modern molecular analysis. The goal of this study is to identify a potential target protein that could act as a predictive factor for effective diagnosis and aid in the development of therapeutic strategies for the treatment of OAB syndrome. We produced OAB in a rat model and performed the first proteomic analysis on the mucosal layer (urothelium) of the bladders of normal and OAB rats.

2018-02-09 | PXD007571 | Pride

Transcriptomic analysis of mouse spermatogonia

Project description:Microarray analysis was performed in order to detail the gene expression profiles in murine b-2M-SPa-6+c-kit-undifferentiated and b-2M-SPa-6+c-kit+ differentiating spermatogonia. These data were used to compare human and mouse transcriptomes of undifferentiated spermatogonia.

2020-08-01 | GSE155507 | GEO

Identifying new diagnostic and treatment pathways for patients with unclassifiable sarcomas

Project description:Whole genome sequencing of tumour normal pairs of human undifferentiated sarcomas.

| EGAS00001001604 | EGA

EGAS00001001604-sc-20170309 - samples

Project description:Whole genome sequencing of tumour normal pairs of human undifferentiated sarcomas.

| EGAD00001003216 | EGA