Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Human Preadipocyte Response to PCB52 or 4-OH-PCB52

ABSTRACT: Human Preadipocyte Response to PCB52 or 4-OH-PCB52

PROVIDER: PRJNA847891 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR19614700_1.fastq.gz	Fastqsanger.gz
	SRR19614700_2.fastq.gz	Fastqsanger.gz
	SRR19614701_1.fastq.gz	Fastqsanger.gz
	SRR19614701_2.fastq.gz	Fastqsanger.gz
	SRR19614702_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 70

Similar Datasets

Human Preadipocyte Response to PCB126

Project description:Human Preadipocyte Response to PCB126

| PRJNA796625 | ENA

Human Preadipocyte Response to PCB52 or 4-OH-PCB52

Project description:Purpose: The goal of this study was to perform an expression transcript profile over a timecourse of human preadipocytes that were treated with PCB52 or 4-OH-PCB52; Methods: Immortal human preadipocytes (NPAD) were treated with 10 uM PCB52 or 4-OH-PCB52 in PGM2 media containing 10%FBS for 9 hrs, 1 day, or 3 days. RNA was extracted with TriZol, DNase treated, and purified using a Qiagen column. RNAseq was used to assess gene expression. Bioinformatic analyses were used to determine differential gene expression between PCB52 or 4-OH-PCB52 treated and vehicle-treated at the same timepoint.

2023-02-13 | GSE205813 | GEO

Imortalized mouse preadipocyte response to Il13 pre-treatment

Project description:Imortalized mouse preadipocyte response to Il13 pre-treatment

| PRJNA720306 | ENA

Primary human preadipocyte differentiation using bulk RNA-sequencing

Project description:Primary human preadipocyte differentiation using bulk RNA-sequencing

| PRJNA1047650 | ENA

Primary human preadipocyte differentiation using bulk RNA-sequencing

Project description:We performed bulk RNA-sequencing on primary human preadipocytes in which we induced differentiation, and collected samples at 6 timepoints across 14 days. The purpose of our study was to study gene expression during the course of preadipocyte differentiation into adipocytes.

2024-01-18 | GSE249195 | GEO

RNA-Seq: The transcriptional response to 1,25(OH)2D3 in human colonic organoids

Project description:RNA-Seq: The transcriptional response to 1,25(OH)2D3 in human colonic organoids

| PRJNA849560 | ENA

microRNA expression in primary human muscle cells treated with 1,25(OH)2D3 or vehicle

Project description:We report the effects of 1,25(OH)2D3 treatment on the microRNA expression in human muscle cells

2015-11-24 | GSE70933 | GEO

Genomic and epigenomic 1α,25(OH)2D3 responses in human colonic organoids

Project description:Background & Aims: Active vitamin D, 1α,25(OH)2D3, is a nuclear hormone with roles in colonic homeostasis and carcinogenesis, yet mechanisms underlying these effects are incompletely understood. Human organoids are an ideal system to study genomic and epigenomic host-environment interactions. Here, we utilize human colonic organoids to measure 1α,25(OH)2D3 responses on genome-wide gene expression and chromatin accessibilityover time. Methods: Human colonic organoids were cultured and treated in triplicate with either 100nM 1α,25(OH)2D3 or vehicle control for 4 and 18 hours (h) for chromatin accessibility, and 6 and 24hfor gene expression. DNA and RNA were extracted for ATAC- and RNA-sequencing, respectively. Differentially accessible peaks were analyzed using DiffBind and EdgeR; differentially expressed genes were analyzed using DESeq2. Motif enrichment was determined using HOMER. Results: At 6h and 24h, 2870 and 2721 differentially expressed genes, respectively (FDR<5%) were identified with overall stronger responses with 1α,25(OH)2D3. Similarly, 1α,25(OH)2D3 treatment led to stronger chromatin accessibility especially at 4h. The vitamin D receptor (VDR) motif was strongly enriched among open chromatin peaks with 1α,25(OH)2D3 treatment accounting for 30.5% and 11% of target sequences at 4h and 18h, respectively (FDR<1%). A number of genes such as CYP24A1, FGF19, MYC, FOS and TGFBR2 showed significant transcriptional and chromatin accessibility responses to 1α,25(OH)2D3 treatment with open chromatin located distant from promoters for some gene regions. Conclusions: Assessment of chromatin accessibility and transcriptional responses to 1α,25(OH)2D3 yielded new observations about vitamin D genome-wide effects in the colon facilitated by application of human colonic organoids. This framework can be applied to study host-environment interactions between individuals and populations in future.

2020-12-09 | GSE162856 | GEO

Monocyte responses to Al(OH)3 adjuvant

Project description:Monocytes, derived from three different donors, were stimulated with the vaccine adjuvant Al(OH)3 for 24 and 48 hours. Proteins were isolated and equal protein amounts were labeledusing TMT(6). We found that Al(OH)3 activates various pathways related to the innate immune response.

2018-03-27 | PXD008452 | Pride

Transcription profiling of human H295R adrenocortical carcinoma cells to investigate OH-PBDE-induced gene expression

Project description:Affymetrix high-density oligonucleotide microarray analysis was performed to analyse hydroxylated polybrominated diphenyl ethers (OH-PBDE)-induced gene expression changes in H295R adrenocortical carcinoma cells. Cells were treated with growth medium in the presence or absence of 10 µM 2-OH-BDE47 or 2-OH-BDE85 for 24 hours before the gene expression changes were investigated. Experiment Overall Design: Gene expression changes in response to hydroxylated polybrominated diphenyl ethers (OH-PBDEs) were analysed by Microarray technology in H295R adrenocortical carcinoma cells. Cells were treated with 10 µM of 2-OH-BDE47 or 2-OH-BDE85 (or growth medium alone) for 24 hours before the OH-PBDE-induced gene expression changes were investigated. Control, 2-OH-BDE47- and 2-OH-BDE85-treated samples were collected from three independent experiments each.

2009-01-02 | E-GEOD-8588 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data