Dataset Information

Monocyte responses to Al(OH)3 adjuvant

ABSTRACT: Monocytes, derived from three different donors, were stimulated with the vaccine adjuvant Al(OH)3 for 24 and 48 hours. Proteins were isolated and equal protein amounts were labeledusing TMT(6). We found that Al(OH)3 activates various pathways related to the innate immune response.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Monocyte, Blood

SUBMITTER: Sietske Kooijman

LAB HEAD: Hugo Meiring

PROVIDER: PXD008452 | Pride | 2018-03-27

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	F1_20150202_SK_FM_monocytesDonor_C1_SCX18-20minTMT126_127med_alum.raw	Raw
	F1_20150202_SK_FM_monocytes_Donor_C1_SCX20-24minTMT126_127med_alum.raw	Raw
	F1_20150202_SK_FM_monocytes_Donor_C1_SCX20-24minTMT126_127med_alum_150204151900.raw	Raw
	F1_20150202_SK_FM_monocytes_Donor_C1_SCX24-26minTMT126_127med_alum.raw	Raw
	F1_20150202_SK_FM_monocytes_Donor_C1_SCX26-28minTMT126_127med_alum.raw	Raw

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Publications

Novel identified aluminum hydroxide-induced pathways prove monocyte activation and pro-inflammatory preparedness.

Kooijman Sietske S Brummelman Jolanda J van Els Cécile A C M CACM Marino Fabio F Heck Albert J R AJR Mommen Geert P M GPM Metz Bernard B Kersten Gideon F A GFA Pennings Jeroen L A JLA Meiring Hugo D HD

Journal of proteomics 20180106

Aluminum-based adjuvants are the most widely used adjuvants in human vaccines. A comprehensive understanding of the mechanism of action of aluminum adjuvants at the molecular level, however, is still elusive. Here, we unravel the effects of aluminum hydroxide Al(OH)<sub>3</sub> by a systems-wide analysis of the Al(OH)<sub>3</sub>-induced monocyte response. Cell response analysis by cytokine release was combined with (targeted) transcriptome and full proteome analysis. Results from this comprehen ...[more]

PMID: 29317357

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