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Comparative analysis of murine Th17 cell transcriptomes in the presence of Smo inhibitor cyclopamine or carrier control

ABSTRACT: Comparative analysis of murine Th17 cell transcriptomes in the presence of Smo inhibitor cyclopamine or carrier control

PROVIDER: PRJNA847939 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19621815.fastq.gz	Fastqsanger.gz
	SRR19621816.fastq.gz	Fastqsanger.gz
	SRR19621817.fastq.gz	Fastqsanger.gz
	SRR19621818.fastq.gz	Fastqsanger.gz
	SRR19621819.fastq.gz	Fastqsanger.gz

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Comparative analysis of murine Th17 cell transcriptomes in the presence of Smo inhibitor cyclopamine or carrier control

Project description:T helper 17 (Th17) cells play a key role in barrier protection against fungal and bacterial pathogens but are also pathological drivers of many inflammatory diseases. We find that Hh signaling inhibition through Smoothened inhibitor cyclopamine selectively inhibits Th17 lineage differentiation but not the development of Th1, Th2, or iTreg CD4+ Th cells. In these experiments we analysed the transcriptomes of Th17 cells that had been polarized in the presence of cyclopamine or carrier control to profile the global transcriptome of Th17 cells in which the Hh pathway had been blocked. The aim of this analysis was to identify the target genes downstream of Smo that may be important for Th17 polarisation.

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Carrier Subclass

Project description:Carrier Subclass

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Whole Retina RNAseq after injury and Cyclopamine(C) treatment in zebrafish

Project description:several genes are being regulated after injury at various time points and after cyclopamine treatment.

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Analysis of Th17 gene signature in the presence of CD28 costimulation in human CD4 naïve T cells

Project description:We determined differentially regulated gene expression profile in CD4 naïve T cells cultured in presence or absence of Th17 inducing cytokines (IL23+IL1β) and anti-CD28. We reported that CD28 costimulation induced Th17-suppressive gene signature.

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Expression data from TH17 cells cultured in the presence or absence of glutamine

Project description:T cell fate and function is closely related to nutrient uptake and utilization. Here we demonstrate that glutamine deprivation switch Th17 cells to Treg cells in a time and dose dependent manner. The finding highlight the critical role of glutamine in T cell fate determination through a metabolic-epigenetic axis. We performed microarrays to explore the gene expression pattern of TH17 cells in the presence or absence of glutamine.

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