Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The Mediator complex regulates enhancer-promoter interactions [Cut&Tag]

ABSTRACT: The Mediator complex regulates enhancer-promoter interactions [Cut&Tag]

PROVIDER: PRJNA848214 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR19631168_1.fastq.gz	Fastqsanger.gz
	SRR19631168_2.fastq.gz	Fastqsanger.gz
	SRR19631169_1.fastq.gz	Fastqsanger.gz
	SRR19631169_2.fastq.gz	Fastqsanger.gz
	SRR19631170_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

The Mediator complex regulates enhancer-promoter interactions [Cut&Tag]

Project description:Enhancer-mediated gene activation generally requires physical proximity between enhancers and their target gene promoters. However, the molecular mechanisms by which interactions between enhancers and promoters are formed are not well understood. Here, we investigate the function of the Mediator complex in the regulation of enhancer-promoter interactions, by combining rapid protein depletion and high-resolution MNase-based chromosome conformation capture approaches. We show that depletion of Mediator leads to reduced enhancer-promoter interaction frequencies, which are associated with a strong decrease in gene expression. In addition, we find increased interactions between CTCF-binding sites upon Mediator depletion. These changes in chromatin architecture are associated with a re-distribution of the Cohesin complex on chromatin and a reduction in Cohesin occupancy specifically at enhancers. Our results indicate that enhancer-promoter interactions are dependent on an interplay between the Mediator and Cohesin complexes and provide new insights into the molecular mechanisms by which communication between enhancers and promoters is regulated.

2023-04-13 | GSE205905 | GEO

Mediator Subunit 30 Directs Myc Oncogenesis [Cut&Tag]

Project description:Mediator Subunit 30 Directs Myc Oncogenesis [Cut&Tag]

2023-11-08 | GSE217545 | GEO

Mediator Subunit 30 Directs Myc Oncogenesis [Cut&Tag]

Project description:Mediator Subunit 30 Directs Myc Oncogenesis [Cut&Tag]

| PRJNA899522 | ENA

The Mediator complex regulates enhancer-promoter interactions

Project description:The Mediator complex regulates enhancer-promoter interactions

| PRJNA848747 | ENA

RNA polymerase II and PARP1 shape enhancer-promoter contacts [CUT&Tag]

Project description:RNA polymerase II and PARP1 shape enhancer-promoter contacts [CUT&Tag]