Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of depletion of histone acetyltransferases and histone deacetylases on gene expression in Drosophila embryos

ABSTRACT: Effect of depletion of histone acetyltransferases and histone deacetylases on gene expression in Drosophila embryos

PROVIDER: PRJNA853323 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR19867400_1.fastq.gz	Fastqsanger.gz
	SRR19867400_2.fastq.gz	Fastqsanger.gz
	SRR19867401_1.fastq.gz	Fastqsanger.gz
	SRR19867401_2.fastq.gz	Fastqsanger.gz
	SRR19867402_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 124

Similar Datasets

Effect of depletion of histone acetyltransferases and histone deacetylases on gene expression in Drosophila embryos

Project description:To understand the impact of histone acetyltransferases and histone deacetylases during ZGA (stage 5) and before ZGA (stage 2), maternally deposited mRNAs were specifically targeted and transcriptomes were interrogated in their absence We then performed similar experiments in stage 5 embryos upon Gcn5 and Nejire KD, rescued with either a wild type or a catalytic dead version of the HAT

2023-04-26 | GSE207018 | GEO

Global and specific responses of the histone acetylome to systematic perturbation

Project description:Regulation of histone acetylation is fundamental to the utilisation of eukaryotic genomes in chromatin. Aberrant acetylation contributes to disease and can be clinically combated by inhibiting the responsible enzymes. Our knowledge of the histone acetylation system is patchy because we so far lacked the methodology to describe acetylation patterns and their genesis by integrated enzyme activities. We devised a generally applicable, mass spectrometry-based strategy to precisely and accurately quantify combinatorial modification motifs. This was applied to generate a comprehensive inventory of acetylation motifs on histones H3 and H4 in Drosophila cells. Systematic depletion of known or suspected acetyltransferases and deacetylases revealed specific alterations of histone acetylation signatures, established enzyme-substrate relationships and unveiled an extensive crosstalk between neighboring modifications. Unexpectedly, overall histone acetylation levels remained remarkably constant upon depletion of individual acetyltransferases. Conceivably, the acetylation level is adjusted to maintain the global charge neutralisation of chromatin and the stability of nuclei.

2015-01-09 | PXD001394 | Pride

Dosage dependent tumor suppression by histone deacetylases 1/2

Project description:Dosage dependent tumor suppression by histone deacetylases 1/2

2013-03-21 | E-MTAB-1448 | biostudies-arrayexpress

Class I histone deacetylases HDAC1, 2 and 3 are histone decrotonylases

Project description:Class I histone deacetylases HDAC1, 2 and 3 are histone decrotonylases

| PRJNA378641 | ENA

Class I histone deacetylases HDAC1, 2 and 3 are histone decrotonylases

Project description:We characterize histone crotonylation in intestinal epithelium-derived cells through Mass spectrometry, ChIp-Seq and RNA-Seq approaches and show that this modification is removed by class I histone deacetylases, HDAC1, 2 and 3.

2017-12-16 | GSE96035 | GEO

Function of histone deacetylases on fission yeast heterochromatin assembly

Project description:To investigate the role of histone deacetylases on heterochromatin assembly, we collected H3K9me2 samples on various HDAC deletion backgrounds.

2024-01-01 | GSE222899 | GEO

Zebrafish embryo Kac - Global proteomic analysis of lysine acetylation in zebrafish (Danio rerio) embryos

Project description:Zebrafish (Danio rerio) model system have used widespread vertebrate investigations for genetic and cell biological analyses, and is suitable for small molecular screens such as chemical, toxicity and drug in order to use for human diseases and drug discovery . Recently, These powerful zebrafish model increasingly apply to human metabolic disease such as obesity and diabetes and toxicology. Despite a lot of advantages, proteomics research at zebrafish has received little interest in comparison with genetic and biological research using histology and in situ hybridization. Protein lysine acetylation is one of the most known post-translational modifications with dynamic and reversibly controlled by lysine acetyltransferase such as histone acetyltransferases and lysine deacetylase such as histone deacetylases and sirtuins family.Also, during the past year, global lysine acetylome studies using MS-based proteomics approach was in diverse species such as human, mouse, E. coli, Yeast and plants. Based on global acetylome data, our understanding of the roles of lysine acetylation in various cellular processes has increased. . The aim of this study was to identify Lysine acetylation in zebrafish embryos and determine the homology from Human at modified site level. Here we showed the global lysine acetylation study in Zebrafish embryos using MS-based zebrafish embryos.

2016-10-10 | PXD003455 | Pride

Histone deacetylases positively regulate transcription through the elongation machinery

Project description:Histone deacetylases positively regulate transcription through the elongation machinery

| PRJEB9544 | ENA

Histone deacetylases positively regulate transcription through the elongation machinery

Project description:Histone deacetylases positively regulate transcription through the elongation machinery

| PRJEB9547 | ENA

Function of histone deacetylases on fission yeast heterochromatin assembly

Project description:Function of histone deacetylases on fission yeast heterochromatin assembly

| PRJNA923801 | ENA