Proteomics

Dataset Information

0

Global and specific responses of the histone acetylome to systematic perturbation


ABSTRACT: Regulation of histone acetylation is fundamental to the utilisation of eukaryotic genomes in chromatin. Aberrant acetylation contributes to disease and can be clinically combated by inhibiting the responsible enzymes. Our knowledge of the histone acetylation system is patchy because we so far lacked the methodology to describe acetylation patterns and their genesis by integrated enzyme activities. We devised a generally applicable, mass spectrometry-based strategy to precisely and accurately quantify combinatorial modification motifs. This was applied to generate a comprehensive inventory of acetylation motifs on histones H3 and H4 in Drosophila cells. Systematic depletion of known or suspected acetyltransferases and deacetylases revealed specific alterations of histone acetylation signatures, established enzyme-substrate relationships and unveiled an extensive crosstalk between neighboring modifications. Unexpectedly, overall histone acetylation levels remained remarkably constant upon depletion of individual acetyltransferases. Conceivably, the acetylation level is adjusted to maintain the global charge neutralisation of chromatin and the stability of nuclei.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Cell Culture, Early Embryonic Cell

SUBMITTER: Ignasi Forne  

LAB HEAD: Prof. Dr. Peter Becker

PROVIDER: PXD001394 | Pride | 2015-01-09

REPOSITORIES: pride

Dataset's files

Source:
Action DRS
ATAC2_1_1_A.raw Raw
ATAC2_1_2_A.raw Raw
ATAC2_1_B.raw Raw
ATAC2_1_D.raw Raw
ATAC2_2_1_A.raw Raw
Items per page:
1 - 5 of 217
altmetric image

Publications

Global and specific responses of the histone acetylome to systematic perturbation.

Feller Christian C   Forné Ignasi I   Imhof Axel A   Becker Peter B PB  

Molecular cell 20150108 3


Regulation of histone acetylation is fundamental to the utilization of eukaryotic genomes in chromatin. Aberrant acetylation contributes to disease and can be clinically combated by inhibiting the responsible enzymes. Our knowledge of the histone acetylation system is patchy because we so far lacked the methodology to describe acetylation patterns and their genesis by integrated enzyme activities. We devised a generally applicable, mass spectrometry-based strategy to precisely and accurately qua  ...[more]

Similar Datasets

2020-10-26 | GSE110287 | GEO
2021-06-29 | PXD021943 | Pride
2018-08-27 | PXD010424 | Pride
2014-07-10 | E-GEOD-57040 | biostudies-arrayexpress
2024-05-20 | GSE260923 | GEO
2024-05-20 | GSE260922 | GEO
2009-08-20 | E-GEOD-15735 | biostudies-arrayexpress
2020-03-26 | PXD015380 | Pride
2023-02-24 | PXD018940 | Pride
2020-02-25 | PXD017054 | Pride