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Intrauterine Botulinum Toxin A administration promotes thin endometrium regeneration in a manner dependent on regulatory effect of insulin-like growth factor-binding protein 3 on osteopontin proteolytic cleavage in mice

ABSTRACT: Intrauterine Botulinum Toxin A administration promotes thin endometrium regeneration in a manner dependent on regulatory effect of insulin-like growth factor-binding protein 3 on osteopontin proteolytic cleavage in mice

PROVIDER: PRJNA854944 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19925166.fastq.gz	Fastqsanger.gz
	SRR19925167.fastq.gz	Fastqsanger.gz
	SRR19925168.fastq.gz	Fastqsanger.gz
	SRR19925169.fastq.gz	Fastqsanger.gz
	SRR19925170.fastq.gz	Fastqsanger.gz

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Intrauterine Botulinum Toxin A administration promotes thin endometrium regeneration in a manner dependent on regulatory effect of insulin-like growth factor-binding protein 3 on osteopontin proteolytic cleavage in mice

Project description:Intrauterine administration of BoTA might be a potential therapeutic strategy to promote endometrial regeneration via elevation of insulin-like growth factor binding protein 3 mediated osteopontin proteolytic cleavage

2022-07-15 | GSE207379 | GEO

Intrauterine botulinum toxin A administration promotes endometrial regeneration mediated by IGFBP3-dependent OPN proteolytic cleavage in thin endometrium.

Project description:Adequate endometrial growth is a critical factor for successful embryo implantation and pregnancy maintenance. We previously reported the efficacy of intrauterine administration of botulinum toxin A (BoTA) in improving the endometrial angiogenesis and the rates of embryo implantation. Here, we further evaluated its potent therapeutic effects on the uterine structural and functional repair and elucidated underlying molecular regulatory mechanisms. This study demonstrated that a murine model of thin endometrium was successfully established by displaying dramatically decreased endometrial thickness and the rates of embryo implantation compared to normal endometrium. Interestingly, the expressions of insulin-like growth factor binding protein-3 (IGFBP3) and an active 35 kDa-form of osteopontin (OPN) were significantly reduced in thin endometrium, which were almost fully restored by intrauterine BoTA administration. Neutralization of BoTA-induced IGFBP3 subsequently suppressed proteolytic cleavage of OPN, exhibiting un-recovered endometrial thickness even in the presence of BoTA administration, suggesting that BoTA-induced endometrial regeneration might be mediated by IGFBP3-dependent OPN proteolytic cleavage. Our findings suggest that intrauterine BoTA administration improves the endometrial environment in our murine model with thin endometrium by increasing endometrial receptivity and angiogenesis in a manner dependent on the regulatory effect of IGFBP3 on OPN proteolytic cleavage, proposing BoTA as an efficient therapeutic strategy for the patients with thin endometrium.

| S-EPMC9816300 | biostudies-literature

Integrated Transcriptomic Analysis of the miRNA-mRNA Interaction Network in Thin Endometrium [miRNA-seq]

Project description:Although the thin endometrium (TE) has been widely recognized as a critical factor in implantation failure, the contribution of miRNA-mRNA regulatory network to the development of disease aetiology remains to be further elucidated. This study performed an integrative analysis of the miRNA-mRNA expression profiles in the thin and adjacent normal endometrium of 8 patients with intrauterine adhesion to construct the transcriptomic regulatory networks. A total of 1093 differentially expressed genes (DEGs) and 72 differentially expressed miRNAs (DEMs) were identified in the thin adhesive endometrium of the TE group compared with the control adjacent normal endometrial cells. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses showed that DEGs and the target genes of DEM were significantly enriched in angiogenesis, cell growth regulation and Wnt signalling pathway. Multiple hub genes (CAV1, MET, MAL2, has-mir-138, ARHGAP6, CLIC4, RRAS, AGFG1, has-mir-200, and has-mir-429) were identified by constructing the miRNA-mRNA regulatory networks. Furthermore, a miRNA-mRNA-pathway-function analysis was conducted, and the hub genes were enriched in the FoxO signalling pathway, cell growth regulation, inflammatory response regulation and regulation of autophagy pathways. Our study is the first to perform integrated mRNA-seq and miRNA-seq analyses in the thin adhesive endometrium and the control adjacent normal endometrial cells. This study provides new insights into the molecular mechanisms underlying the formation of thin endometrium.

2021-04-07 | GSE160634 | GEO

Integrated Transcriptomic Analysis of the miRNA-mRNA Interaction Network in Thin Endometrium [RNA-Seq]

2021-04-07 | GSE160633 | GEO

Effect of growth hormone on thin endometrium via intrauterine infusion.

Project description:BackgroundA thin endometrium has become a common reason for the repeated implantation failure. Growth hormone (GH) can regulate the proliferation and metabolism of endometrial cells. The aim of this study was to explore the effect of GH on thin endometrium.MethodsA total of 48 female Sprague-Dawley (SD) rats were randomly assigned to the following 4 groups with 12 rats in each group: blank control, model, subcutaneous, and GH groups. The blank control group was untreated and maintained in a routine manner. The model, subcutaneous, and GH groups were intrauterine perfused with 95% ethanol during estrus. After 6-8 h, the model group was intrauterine perfused with 0.2 mL normal saline, the subcutaneous group received subcutaneous injection of 0.12 mg/kg GH dissolved in 0.2 mL normal saline, and the GH group was intrauterine perfused with 0.12 mg/kg GH dissolved in 0.2 mL normal saline. Hematoxylin and eosin (HE) staining was used to examine the thickness of the endometrium. The expression of cytokeratin and vimentin was detected by western blotting and immunohistochemistry.ResultsThe intima thickness in the GH group and blank control group was increased compared with that in the model group (P<0.01). The intima thickness in the subcutaneous group was increased compared with that in the model group, but there was no significant difference. The expression of vimentin and cytokeratin was increased in the GH (P<0.01) and blank control (P<0.01) groups compared with that in the model group.ConclusionsIntrauterine perfusion of GH can promote the regeneration and repair of thin endometrium in rats. The therapeutic effect of uterine infusion of GH is better than that of subcutaneous injection of GH.

| S-EPMC8422125 | biostudies-literature

Effect of intrauterine MGO administration on bovine endometrium

Project description:To clarify the effect of intrauterine methylglyoxal (MGO) administration on the endometrium, mRNA expression profiles of bovine endometrium were investigated. Hierarchical cluster analysis with the expression levels of all genes was divided these cows into two clusters. First cluster was composed of control cows, second cluster contained MGO (5 mM) treated cows.

2021-01-19 | GSE165039 | GEO

Effect of intrauterine MGO administration on bovine endometrium

Project description:Effect of intrauterine MGO administration on bovine endometrium

| PRJNA693017 | ENA

Osteopontin vs empty vector

Project description:Osteopontin tranfected vs empty vector breast cell line Keywords: parallel sample

2004-01-07 | GSE939 | GEO

Global Gene Expression Fails to Discriminate Thin Melanomas with Poor Outcomes

Project description:Melanoma is the fifth-most common cancer diagnosed in the United States with a dramatic increase in incidence among both men and women. Most melanomas present as thin lesions (≤1.0mm) and have a good prognosis. However a small percentage of patients (5-10%) with thin lesions recur or metastasize. The aim of our study was to elucidate which thin melanomas (< 1.0 mm thickness) have the propensity to metastasize to regional lymph nodes or distant sites. Our focus was analyzing gene expression as a whole. We sought to identify a distinct pattern of gene expression within thin melanomas that were known to have eventually metastasized to regional lymph nodes or distant sites, from those that followed the typical course with good response to wide local excision alone. While gene expression differences were observed between nine thin melanoma patients with poor clinical outcome compared to ten patients with a good clinical outcome, neither the number of genes nor the magnitude of the fold difference was very substantial or significant. Although cluster analysis was able to use this subset of genes to definitively separate a subset of the poor responders from the good responders, there remained a mixed group of tumors which could not be predicted from gene expression alone. Pathway analysis did identify cellular processes that were regulated based upon response, including categories commonly associated with melanoma progression The goal of this pilot study was to identify gene expression changes within the tumor cells of thin melanomas that have a poor outcome that would discriminate them from thin melanomas that do not recur or metastasize . Our results conclude that there were very few differences between these groups. Future research will be required and investigation of the mutational landscape of these different tumor sub-types may be another strategy to uncover genomic changes that drive recurrence and metastasis.

2017-01-15 | GSE79662 | GEO

Integrated Transcriptomic Analysis of the miRNA-mRNA Interaction Network in Thin Endometrium

Project description:Integrated Transcriptomic Analysis of the miRNA-mRNA Interaction Network in Thin Endometrium

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