Layer chickens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA855329 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Osteoporosis is a major problem for the laying hen industry due to welfare issues and economic losses related to bone fractures. The objective of this research was to better understand genetic influences on bone integrity by comparing gene expression profiles from bone marrow of chickens expressing phenotypic differences for a number of bone characteristics. Global expression profiles of genes included on the Fred Hutchinson Cancer Research Center (FHCRC) Chicken 13K array were assessed in broiler and layer chickens. Keywords: Gene expression comparison between two chicken lines at 15 weeks of age
2007-01-01 | GSE6268 | GEO
Project description:Osteoporosis is a major problem for the laying hen industry due to welfare issues and economic losses related to bone fractures. The objective of this research was to better understand genetic influences on bone integrity by comparing gene expression profiles from bone marrow of chickens expressing phenotypic differences for a number of bone characteristics. Global expression profiles of genes included on the Fred Hutchinson Cancer Research Center (FHCRC) Chicken 13K array were assessed in broiler and layer chickens. Keywords: Gene expression comparison between two chicken lines at 60 weeks of age
2007-01-01 | GSE6270 | GEO
Project description:To realize the gene expression in response to acute heat stress in chicken small yellow follicles, we have employed whole genome microarray expression profiling as we have employed whole genome microarray expression profiling as a tool to identify genes response to acute heat stress. Female B strain Taiwan country chickens were subjected to acute heat stress (38℃) for 2 h, and then exposed to 25℃, with small yellow follicles collected 0, 2, and 6 h after the cessation of heat stress, using non heat-stressed hens as a control group (n = 3 hens per group). Based on a chicken 44K oligo microarray, 69, 51, and 76 genes were upregulated and 58, 15, 56 genes were downregulated after heat treatment of H2R0, H2R2, and H2R6, respectively, using a cutoff value of two-fold or higher in the small yellow follicles of the heat-stressed chickens from those of the control chickens. Upregulation of heat shock protein 25, interleukin 6, metallopeptidase 1, and metalloproteinase 13, and downregulation of type II alpha 1 collagen, discoidin domain receptor tyrosine kinase 2, and Kruppel-like factor 2 were confirmed through quantitative real-time polymerase chain reaction (qRT-PCR).
2017-05-20 | GSE99108 | GEO
Project description:A study of a marked QTL affecting body weight segregating in an inter-cross of a broiler and layer chickens. QTL genotypes were inferred on flanking markers and RNA from breast tissue of birds with alternative QTL genotypes was hybridized onto microarrays
2010-07-20 | E-TABM-475 | biostudies-arrayexpress
Project description:ISA Brown Warren medium heavy layer hens were divergently selected for 25 generations on their primary immune response. Two selection chicken lines were established: chickens with a high natural antibody response (H) and chicken with a low natural antibody response (L). Besides a control line of randomly bred chickens was included (C) (Parmentier et al.
2010-03-02 | E-MEXP-1798 | biostudies-arrayexpress
Project description:In this study we investigated the methylome of chickens immunized with Infectious laryngotracheitis (ILT) vaccine derived from chicken embryos. Methyl-CpG binding domain protein-enriched genome sequencing (MBD-Seq) method was employed in the detection of the 1,155 differentially methylated regions (DMRs) across the entire genome. After validation, we ascertained the genomic DMRs distribution and annotated them regarding genes, transcription start sites (TSS) and CpG islands. We found that global DNA methylation decreased in vaccinated birds, presenting 704 hypomethylated and 451 hypermethylated DMRs, respectively. Additionally, we performed an enrichment analysis detecting gene networks, in which cancer and RNA post-transcriptional modification appeared in the first place, followed by humoral immune response, immunological disease and inflammatory disease. The top four identified canonical pathways were EIF2 signaling, regulation of EIF4 and p70S6K signaling, axonal guidance signaling and mTOR signaling, providing new insight regarding the mechanisms of ILT etiology. Lastly, the association between DNA methylation and differentially expressed genes was examined, and detected negative correlation in seventeen of the eighteen genes. DNA methylation analysis employing MBD-Seq with 3 salt concentrations, in vaccinated and control group of chickens with 2 biological replications
2014-08-28 | E-GEOD-56984 | biostudies-arrayexpress
Project description:Intestinal microbiota of broiler chickens Genome sequencing and assembly
| PRJNA1178860 | ENA
Project description:A deletion mutation in the growth hormone receptor (GHR) gene results in the inhibition of skeletal muscle growth and fat deposition in dwarf chickens. In this study, microarray techniques were used to detect the miRNA and mRNA expression profiles of 14-day-old embryo and 7-week-old chicken skeletal muscle of deletion-type dwarf chickens and normal-type chickens. Skeletal muscle tissues of Dwarf recessive White Rock chickens and normal recessive White Rock chickens were used to make the microarray assay. Results show the expression of miR-1623 and miR-181b in 14-day-old embryos and of let-7b and miR-128 in 7-week-old chickens. let-7b was the only miRNA found to be completely complementary to its target in the 3'UTR of GHR and inhibited GHR gene expression. KEGG (Kyoto Encyclopaedia of Genes and Genomes) pathway analysis and RT-PCR verified that there were three main signalling pathways regulating the skeletal muscle growth and fat deposition of chickens influenced by the let-7b-regulated GHR gene. The suppression of the cytokine signalling 3 (SOCS3) gene was found to be involved in the signalling pathway of adipocytokines. We found that let-7b is the critical miRNA involved in the regulation of the GHR gene. SOCS3 plays a critical role in the network regulating skeletal muscle growth and fat deposition via let-7b-mediated GHR gene expression.
2012-04-18 | GSE37367 | GEO