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Whole-transcriptome sequencing and ceRNA interaction network construction for temporomandibular joint osteoarthritis [miRNA-Seq]

ABSTRACT: Whole-transcriptome sequencing and ceRNA interaction network construction for temporomandibular joint osteoarthritis [miRNA-Seq]

PROVIDER: PRJNA855870 | ENA |

REPOSITORIES: ENA

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Whole-transcriptome sequencing and ceRNA interaction network construction for temporomandibular joint osteoarthritis [miRNA-Seq]

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Whole-transcriptome sequencing and ceRNA interaction network construction for temporomandibular joint osteoarthritis [RNA-Seq]

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Project description:To investigate differentially expressed lncRNAs,circRNAs,miRNAs and mRNAs in synovium of human temporomandibular joint osteoarthritis (TMJOA), we performed RNA high-throughput sequencing in synovium of human TMJOA. We then performed gene expression profiling analysis using data obtained from RNA-seq .

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Effects of mechanical stress and deficiency of dihydrotestosterone or 17β-estradiol on temporomandibular joint osteoarthritis in mice

Project description:Temporomandibular joint osteoarthritis (TMJ-OA), a subtype of temporomandibular joint dysfunction (TMD), is characterized by progressive cartilage degradation, subchondral bone erosion, and chronic pain. Although there has been extensive research on TMJ-OA, its etiology remains unknown. Age, hormonal factors, and excessive mechanical stress on the TMJ are proposed risk factors for TMJ-OA. Using microarrays, we discovered two disease susceptibility genes that have been suggested to be involved in the pathogenic mechanism of TMJ-OA.

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Project description:miRNA sequencing in synovium of human temporomandibular joint osteoarthritis

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Gene Expression Profiling of IL-17A-treated Synovial Fibroblasts from the Human Temporomandibular Joint

Project description:Synovial fibroblasts contribute to the inflammatory temporomandibular joint under pathogenic stimuli. Synovial fibroblasts and T cells participate in the perpetuation of joint inflammation in a mutual activation feedback, via secretion of cytokines and chemokines that stimulate each other. IL-17 is an inflammatory cytokine produced primarily by Th17 cells that plays critical roles in the pathogenesis of numerous autoimmune and inflammatory diseases. Here, we investigated the roles of IL-17A in temporomandibular joint disorders (TMD) by using genome-wide analysis of synovial fibroblasts isolated from patients with TMD. We analyzed the gene expression profiles of synovial fibroblasts that were treated with or without IL-17A. IL-17 induced gene expression in synovial fibroblasts from human temporomandibular joint was measured at 4 hours after treated with IL-17A (10 ng/ml) and untreated control samples. This experiment used one donor sample.

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