Project description:Dictyostelium discoideum is a professional phagocyte and it uses phagocytosis as a way to get food. However, there are a number of bacteria that subvert this professional phagocyte. In this study we investigated whether Salmonella typhimurium is also pathogenic for D. discoideum.
Project description:Dictyostelium discoideum is a professional phagocyte and it uses phagocytosis as a way to get food. However, there are a number of bacteria that subvert this professional phagocyte. In this study we investigated whether Salmonella typhimurium is also pathogenic for D. discoideum.
Project description:Dictyostelium discoideum is a professional phagocyte and it uses phagocytosis as a way to get food. However, there are a number of bacteria that subvert this professional phagocyte. In this study we investigated whether Salmonella typhimurium is also pathogenic for D. discoideum.
Project description:Dictyostelium discoideum is a professional phagocyte and it uses phagocytosis as a way to get food. However, there are a number of bacteria that subvert this professional phagocyte. In this study we investigated whether Salmonella typhimurium is also pathogenic for D. discoideum.
Project description:Dictyostelium discoideum is a professional phagocyte and it uses phagocytosis as a way to get food. However, there are a number of bacteria that subvert this professional phagocyte. In this study we investigated whether Salmonella typhimurium is also pathogenic for D. discoideum.
Project description:Dictyostelium discoideum is a professional phagocyte and it uses phagocytosis as a way to get food. However, there are a number of bacteria that subvert this professional phagocyte. In this study we investigated whether Salmonella typhimurium is also pathogenic for D. discoideum.
Project description:Transcriptomic analysis of dedifferentiation in Dictyostelium discoideum: comparing wild type and forG- (formin-) mutant cells dedifferentiating in growth medium
Project description:Purpose: To filter genes that may contribute to introcellualr survival of B. bronchiseptica inside Dictyostelium discoideum, the genes that differently expressed when bacteria inside amoebae or in culture medium are selected as target genes.
Project description:Dictyostelium discoideum, a microbial model for social evolution, is known to distinguish self from non-self and show genotype-dependent behavior during chimeric development. Aside from a small number of cell-cell recognition genes, however, little is known about the genetic basis of self/non-self recognition in this species. Based on the key hypothesis that there should be differential expression of genes if D. discoideum cells were interacting with non-clone mates, we performed transcriptomic profiling study in this species during clonal vs. chimeric development. Wild strains isolated from North Carolina, which have been shown to form a dominance hierarchy when co-developing in chimeras, were used. The transcriptomic profiles of D. discoideum cells in clones vs. different chimeras were compared at five different developmental stages using a customized microarray. Effects of chimerism on global transcriptional patterns associated with social interactions were observed. The chimera developmental program may also provide insights on behavioral changes associated with social conflicts in this species.
Project description:Dictyostelium discoideum, a microbial model for social evolution, is known to distinguish self from non-self and show genotype-dependent behavior during chimeric development. Aside from a small number of cell-cell recognition genes, however, little is known about the genetic basis of self/non-self recognition in this species. Based on the key hypothesis that there should be differential expression of genes if D. discoideum cells were interacting with non-clone mates, we performed transcriptomic profiling study in this species during clonal vs. chimeric development. Wild strains isolated from North Carolina, which have been shown to form a dominance hierarchy when co-developing in chimeras, were used. The transcriptomic profiles of D. discoideum cells in clones vs. different chimeras were compared at five different developmental stages using a customized microarray. Effects of chimerism on global transcriptional patterns associated with social interactions were observed. The chimera developmental program may also provide insights on behavioral changes associated with social conflicts in this species.
