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Changes in Treg gene expression upon deletion of Usp22 and Usp21

ABSTRACT: Changes in Treg gene expression upon deletion of Usp22 and Usp21

PROVIDER: PRJNA857304 | ENA |

REPOSITORIES: ENA

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Changes in Treg gene expression upon deletion of Usp22 and Usp21

Project description:Usp22 and Usp21 are important for Foxp3 stability. We used RNA sequencing to determine the broad changes upon Usp22 and Usp21 deletionWe then performed gene expression profiling analysis using data obtained from RNA-seq and compared to each other

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Next Generation Sequencing Facilitates Quantitative Analysis of FOXP3-YFP-Cre and FOXP3-YFP-Cre/USP21-floxp/floxp Treg Cells Transcriptomes

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Project description:Nanog is a master pluripotency factor of embryonic stem cells (ESCs). Stable expression of Nanog is required to maintain the stemness of ESCs, although Nanog is a short-lived protein and quickly degraded by the ubiquitin-dependent proteasome system (UPS). Here, we report that the deubiquitinase USP21 interacts with, deubiquitinates and stabilizes Nanog and therefore maintains the protein level of Nanog in mouse-ESCs (mESCs). Loss of USP21 results in Nanog destruction, mESCs differentiation and reduced the somatic cell reprogramming efficiency. USP21 is a transcriptional target of the LIF/STAT3 pathway and is downregulated upon differentiation. Moreover, differentiation cues promote ERK-mediated phosphorylation and dissociation of USP21 from Nanog, thus leading to Nanog degradation. Additionally, USP21 is recruited to gene promoters by Nanog to deubiquitinate histone H2A at K119 and thus facilitates Nanog-mediated gene expression. Together, our findings provide a regulatory mechanism by which extrinsic signals regulate mESC fate via deubiquitinating Nanog.

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Project description:This analysis identifies genes which are affected in SW480 cells upon siRNA-mediated USP22 knockdown

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