Project description:Primary objectives: The primary objective is to investigate circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
Primary endpoints: circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
Project description:PREMISE OF THE STUDY:Senna spectabilis var. excelsa (Fabaceae) is a South and Central American tree of great ecological importance and one of the most common species in several sites of seasonally dry forests. Our goal was to develop microsatellite markers to assess the genetic diversity and structure of this species. METHODS AND RESULTS:We designed and assessed 53 loci obtained from a microsatellite-enriched library and an intersimple sequence repeat library. Fourteen loci were polymorphic, and they presented a total of 39 alleles in a sample of 61 individuals from six populations. The mean values of observed and expected heterozygosities were 0.355 and 0.479, respectively. Polymorphism information content was 0.390 and the Shannon index was 0.778. CONCLUSIONS:Polymorphism information content and Shannon index indicate that at least nine of the 14 microsatellite loci developed are moderate to highly informative, and potentially useful for population genetic studies in this species.
Project description:The first complete chloroplast genome (cpDNA) sequence of Altingia excelsa was determined from Illumina HiSeq pair-end sequencing data in this study. The cpDNA is 160,861 bp in length, contains a large single copy region (LSC) of 89,126 bp and a small single copy region (SSC) of 19,011 bp, which were separated by a pair of inverted repeats (IR) regions of 26,362 bp each. The genome contains 127 genes, including 82 protein-coding genes, 8 ribosomal RNA genes, and 37 transfer RNA genes. Phylogenomic analysis showed that A. excelsa and Liquidambar formosana clustered in a clade in Saxifragales order.
