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RNA-seq comparing Cushing's disease adenomas and surrounding normal tissues

ABSTRACT: RNA-seq comparing Cushing's disease adenomas and surrounding normal tissues

PROVIDER: PRJNA858401 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR20140286_1.fastq.gz	Fastqsanger.gz
	SRR20140286_2.fastq.gz	Fastqsanger.gz
	SRR20140287_1.fastq.gz	Fastqsanger.gz
	SRR20140287_2.fastq.gz	Fastqsanger.gz
	SRR20140288_1.fastq.gz	Fastqsanger.gz

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RNA-seq comparing Cushing's disease adenomas and surrounding normal tissues

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Project description:This SuperSeries is composed of the SubSeries listed below.

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Project description:Recurrent single-nucleotide and small indel somatic mutations were infrequent among the three adenoma subtypes. However, somatic copy-number alterations (SCNA) were identified in all three pituitary adenoma subtypes. Methylation analysis revealed adenoma subtype-specific DNA methylation profiles, with GHsecreting adenomas being dominated by hypomethylated sites. Likewise, gene-expression patterns revealed adenoma subtype-specific profiles. Integrating DNA methylation and gene-expression data revealed that hypomethylation of promoter regions are related with increased expression of GH1 and SSTR5 genes in GH-secreting adenomas and POMC gene in ACTH secreting adenomas. Finally, multispectral IHC staining of immune-related proteins showed abundant expression of PD-L1 among all three adenoma subtypes.

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