Project description:Chromatogram library generated of pooled sample. Coculture spheroids formed from fibroblast and colon cancer cell lines, and monoculture spheroids formed from the colon cancer cell line HCT116.
Project description:This study aims to to compare the gene transcription profiles of endothelial cells and stem cells, when they are cultured alone or when they are cultured together. Thus there are two major questions - how do the cells differ, and how do the cells influence each other's gene expression. Thus there are 4 types of sample: endothelial cell monoculture, endothelial cell coculture, stem cells monoculture, stem cell coculture. There are also 4 biological replicates (independent experiments) leading to 16 array data files.
Project description:In vivo co-cultures of colitis-associated cancer epithelial organoids alone or with matched proximal colon fibroblasts or with matched colitis-associated cancer fibroblasts
Project description:In the colon, a single-layered epithelium lines the crypts, which descend into the underlying mucosa. Surrounding the crypts lies a large network of fibroblasts with diverse functions, including extracellular matrix (ECM) production and stem cell maintenance. However, functional diversity of those cells remains unexplored. To address fibroblast heterogeneity in colon homeostasis, we conducted single-cell RNA sequencing on epithelium-stripped colonic mucosal samples of aSMACre:mTmG-reporter mouse. We found 6 clusters of fibroblasts.
Project description:The colon cancer associated fibroblasts are part of the tumor microenvironment and has been shown to facilitate colon cancer progression by secretion of various signaling molecules and growth hormones. Similarly, the fibroblasts associated with colitic colons secrete pro-inflammatory cytokines that promote inflammation of the colon. However, the molecular mechanisms that underlie these gene expression changes in these fibroblasts is unclear. To characterize the epigenetic mechanisms that may contribute to the gene expression changes in these fibroblasts, we performed RNA-seq and MBD-isolated genome sequencing (MiGS or MBD-seq) on colon associated fibroblasts isolated from normal, colitis and cancer patients.
Project description:We described differential expression of CD90 in gp38+ fibroblasts in murine colon. We functionally characterized CD90- and CD90+ fibroblasts by co-culturing with intestinal organoids. CD90+ colon fibroblasts were able to support organoid growth while CD90- did not.