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Stabilization of hESCs in two distinct substates along the continuum of pluripotency

ABSTRACT: Stabilization of hESCs in two distinct substates along the continuum of pluripotency

PROVIDER: PRJNA859148 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR20229613.fastq.gz	Fastqsanger.gz
	SRR20229614.fastq.gz	Fastqsanger.gz
	SRR20229615.fastq.gz	Fastqsanger.gz
	SRR20229616.fastq.gz	Fastqsanger.gz
	SRR20229617.fastq.gz	Fastqsanger.gz

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Project description:Stabilization of virgin naïve hESCs in two distinct substates along the continuum of pluripotency [miRNA-seq]

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Stabilization of hESCs in two distinct substates along the continuum of pluripotency.

Project description:A detailed understanding of the developmental substates of human pluripotent stem cells (hPSCs) is needed to optimize their use in cell therapy and for modeling early development. Genetic instability and risk of tumorigenicity of primed hPSCs are well documented, but a systematic isogenic comparison between substates has not been performed. We derived four hESC lines in naive human stem cell medium (NHSM) and generated isogenic pairs of NHSM and primed cultures. Through phenotypic, transcriptomic, and methylation profiling, we identified changes that arose during the transition to a primed substate. Although early NHSM cultures displayed naive characteristics, including greater proliferation and clonogenic potential compared with primed cultures, they drifted toward a more primed-like substate over time, including accumulation of genetic abnormalities. Overall, we show that transcriptomic and epigenomic profiling can be used to place human pluripotent cultures along a developmental continuum and may inform their utility for clinical and research applications.

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Stabilization of hESCs in two distinct substates along the continuum of pluripotency

Project description:This SuperSeries is composed of the SubSeries listed below.

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