Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profile at single cell level of splenic cDC

ABSTRACT: Gene expression profile at single cell level of splenic cDC

PROVIDER: PRJNA859169 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	SRR20239973_1.fastq.gz	Fastqsanger.gz
	SRR20239973_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 3 of 3

Similar Datasets

Gene expression profile at single cell level of splenic cDC

Project description:We used single cell RNA sequencing (scRNA-seq) to analyze different genes between WT splenic cDC and KO splenic cDC.

2023-09-07 | GSE208311 | GEO

Single Cell RNA-Seq Analysis Reveals Fate Decision of Human Pre-cDC Determined by Transcription Factor Competition

Project description:Classic dendritic cells (cDCs) play a central role in the immune system and consist of two major subsets: CD141+ cDC (cDC1) and CD1c+ cDC (cDC2). The pre-cDCs is the immediate precursors to both cDC subsets. Previous studies showed that there were two pre-committed pre-cDC subpopulations. However, the key molecular drivers of pre-commitment in human pre-cDCs were not investigated. To address this question, we performed both single cell and bulk RNA sequencing (RNA-seq) of two cDC subsets and pre-cDCs. We inferred a list of sixteen candidate master regulator transcriptional factors (TFs) that can indeed separate pre-cDCs into two sub-populations, with one close to cDC1 and the other close to cDC2. More importantly, these two pre-cDC sub-populations are correlated with ratio of IRF8 to IRF4 expression level more than their individual expression level. Our results suggest the concept that the ratio of antagonistic TFs and their competition determine cDC subset differentiation fate.

2019-07-16 | GSE89322 | GEO

RNA sequencing of primary isolated unstimulated murine splenic cDC from specific pathogen free (SPF), Germ Free (GF) and Interferon alpha/beta receptor 1 (Ifnar) knock out mice.

Project description:The transcriptome of primary isolated unstimulated murine splenic cDC from specific pathogen free (SPF) was compared to cDC from Germ Free (GF) and Interferon alpha/beta receptor 1 (Ifnar) knock out mice. Cells were isolated and sorted by flow cytometry to high purity. Total RNA was isolated using the RNeasy plus micro kit (Qiagen). Barcoded mRNA seq libraries were prepared from 100 ng of total RNA (RIN>8) using a NEBnext poly(A) mRNA Magnetic Isolation Module and NEBnext UltraII lib prep kit for Illumina (New England Biosciences) according to the manual. Single-end RNA sequencing (59nt) was performed on a HiSeq 2500 (Illumina). Barcoded RNA-seq libraries were onboard clustered using HiSeq® Rapid SR Cluster Kit v2 using 8pM and 59bps were sequenced on the Illumina HiSeq 2500 using HiSeq Rapid SBS Kit v2 (59 Cycle). The raw output data of the HiSeq was preprocessed according to the Illumina standard protocol.

2020-03-30 | E-MTAB-8882 | biostudies-arrayexpress

Gene expression profile at single cell level of thalamus

Project description:Gene expression profile at single cell level of thalamus

| PRJNA942935 | ENA

Transcriptomic profile at single cell level of splenic NK cells from Reg-1fl/fl and Reg-1ΔNK mice [CITE-Seq]

Project description:Transcriptomic profile at single cell level of splenic NK cells from Reg-1fl/fl and Reg-1ΔNK mice [CITE-Seq]

| PRJNA996084 | ENA

Gene expression profile at single cell level of vestibular schwannoma

Project description:Gene expression profile at single cell level of vestibular schwannoma

| PRJNA960863 | ENA

Gene expression profile at single cell level of BPH cells

Project description:Gene expression profile at single cell level of BPH cells

| PRJNA939217 | ENA

Gene expression profile at single cell level of xenograft tumors

Project description:Gene expression profile at single cell level of xenograft tumors

| PRJNA835440 | ENA

Gene expression profile at single cell level of hippocampal organoids

Project description:Gene expression profile at single cell level of hippocampal organoids

| PRJNA1102029 | ENA

Gene expression profile at single cell level of neuroblastoma tumors

Project description:Gene expression profile at single cell level of neuroblastoma tumors

| PRJNA903879 | ENA

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data