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Unravelling the pathobiological role of the fungal KEOPS complex in Cryptococcus neoformans

ABSTRACT: Unravelling the pathobiological role of the fungal KEOPS complex in Cryptococcus neoformans

PROVIDER: PRJNA859376 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR20274736_1.fastq.gz	Fastqsanger.gz
	SRR20274736_2.fastq.gz	Fastqsanger.gz
	SRR20274737_1.fastq.gz	Fastqsanger.gz
	SRR20274737_2.fastq.gz	Fastqsanger.gz
	SRR20274738_1.fastq.gz	Fastqsanger.gz

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Unravelling the pathobiological role of the fungal KEOPS complex in Cryptococcus neoformans

Project description:The kinase, putative endopeptidase and other peptides of small size (KEOPS) complex plays critical cellular functions in eukaryotes, but its pathobiological roles remains elusive in fungal pathogens. Here we comprehensively analyze the pathobiological functions of the KEOPS complex in fungal meningoencephalitis pathogen Cryptococcus neoformans. The cryptococcal KEOPS complex appears to have a linear arrangement of Pcc1-Kae1-Bud32-Cgi121, supported by physical interaction between Pcc1-Kae1, Kae1-Bud32, and Bud32-Cgi121, but does not have Gon7 ortholog, which is the part of the S. cerevisiae KEOPS complex. Deletion of PCC1, KAE1, and BUD32 causes severe growth defects with perturbed cell cycle control.

2022-10-18 | GSE208333 | GEO

Cryptococcus neoformans proteins during fungal-macrophage interaction

Project description:Cryptococcus neoformans interactions with murine macrophages are critical for disease. In this project we analyzed fungal proteins which were co-purified with murine host proteins after interaction. H99 C. neoformans was opsonized with mAb 18B7 and addedd to murine macrophages. Then murine cells were lysed and cell extracts submitted to proteomics.

2023-03-11 | PXD024951 | Pride

Ribosome profiling of Cryptococcus neoformans H99

Project description:We measured protein translation (by ribosome profiling) and RNA levels (by polyA-enriched RNA-seq) in Cryptococcus neoformans strain H99 and Cryptococcus neoformans strain JEC21. This is the first transcriptome-wide map of translation in this species complex.

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CoCl2 Response in Cryptococcus neoformans

Project description:We investigated the effects of the hypoxia-mimetic CoCl2 on the gene expression of pathogenic fungus Cryptococcus neoformans. Keywords: compound treatment design

2009-05-01 | GSE11390 | GEO

Unraveling the Mechanism of Thermotolerance in Cryptococcus neoformans by Set3C Complex

Project description:Thermotolerance, a key factor essential for the virulence of pathogenic fungi including Cryptococcus neoformans, remains largely unexplored in terms of its underlying mechanism. In this study, our findings demonstrate that Set3C, a widely distributed and conserved histone deacetylase complex, is required for thermotolerance in Cryptococcus neoformans. Specifically, the deletion of the core subunit Set302, responsible for the integrity of the complex, results in a significant reduction in the growth ability under high stress and the viability at extreme temperature. Moreover, the absence of Set302 leads to a decrease in the production of capsule and melanin. Transcriptomics analysis revealed that Set302 regulates a large number of genes compared to normal condition, and their expression is responsive to heat stress. Notably, we observed that Set302 positively influences the expression of genes related to ubiquitin-proteasome system (UPS) at high temperature. Using GFP-α-synuclein overexpression model, we observed a pronounced accumulation of misfolded proteins under heat stress, consequently inhibiting the thermotolerance of Cryptococcus neoformans. Furthermore, the loss of Set302 exacerbates this inhibition of thermotolerance. Interestingly, set302∆ strain exhibits a similar phenotype under proteasome stress as it does under high temperature. We also found that set302∆ strain displayed significantly reduced pathogenicity and colonization ability compared to the wild-type strain in the murine infection model. Collectively, our findings indicate that Set302 modulates the degradation of misfolded proteins through the UPS pathway, thereby affecting the thermotolerance and pathogenicity of Cryptococcus neoformans.

2023-09-05 | GSE242109 | GEO

Investigating Conservation of the Cell-Cycle-Regulated Transcriptional Program in the Fungal Pathogen, Cryptococcus neoformans

Project description:Cell-cycle transcript dynamics from two species of wild-type budding yeast growing at 30 degrees Celsius in rich media: Saccharomyces cerevisiae (BF264-15D background) and Cryptococcus neoformans var. grubii (H99F background). We compared programs of cell-cycle-regulated genes between distantly related budding yeasts.

2016-12-07 | GSE80474 | GEO

Global proteomic analysis in Cryptococcus neoformans

Project description:Discovering the Roles of the Casein Kinase 2 Complex in the Growth, Differentiation, Stress Responses, and Pathogenicity of Cryptococcus neoformans

2024-05-23 | PXD033626 | Pride

Light-regulated genes in Cryptococcus neoformans

Project description:Light is a universal environmental signal perceived by many organisms, including the fungi in which light regulates both common and unique biological processes depending on the species. We conducted a whole-genome microarray analysis on the basidiomycete fungus Cryptococcus neoformans to identify light-regulated genes.

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Unravelling of the novel melanin biosynthesis-signalling networks in Cryptococcus neoformans

Project description:Unravelling of the novel melanin biosynthesis-signalling networks in Cryptococcus neoformans

| PRJNA545271 | ENA

Pianalto KM et al "Characterization of novel components of the environmental pH-sensing complex in Cryptococcus neoformans"

Project description:Three analyses performed by the Duke Proteomics and Metabolomics Shared Resource in support of the project entitled "". Sample ID21519 was called Rra1-GFP, a 1D coomassie-stained gel band from a GFP-Trap pulldown of Rra1-GFP from the membrane protein fraction of Cryptococcus neoformans. Sample ID21570 was called no-GFP control, and consisted a GFP-Trap pulldown from the insoluble fraction of C. neoformans H99 lysate (non GFP expressing). ID21571 was called Rra1-GFP, and consisted of a GFP-Trap pulldown from the insoluble fraction of C. neoformans H99 lysate containing Rra1-GFP.

2018-03-06 | MSV000082127 | MassIVE

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