Project description:Polymeric nanoparticles (NPs) have emerged as promising tools for immunomodulation in various disease contexts. The interactions between the NP surface and plasma-resident biomolecules result in the formation of a biomolecular corona (BC), which varies patient-to-patient and as a function of disease state, resulting in the concept of the personalized NP-BC. This study investigates how the progression of systemic inflammatory disease influences the NP-BC composition and its corresponding effects on innate immune cell interactions and activation profiles. Employing a murine model of systemic inflammation, the dynamic changes in plasma biomolecule composition and their corresponding NP-BC fingerprints were elucidated. An integrated multi-omics approach was developed to reveal disease state-specific alterations in innate immune cell phenotypes and modulated signaling pathways to identify and confirm key circulating biomolecules that contribute to the dynamic immunostimulatory capabilities of BCs.
Project description:Whole blood transcriptomics analysis of healthy individuals, immunized with the MVA-B vaccine via transcutaneous or intramuscular route. We define an early gene expression profile, detected at day 1 and day 7 after immunization, that is associated with the development of either MVA specific humoral or CD8 T cell responses. We used System Biology approach to analyse vaccine efficacy in order to find innate predictive biomarkers of the quality of adaptive responses for potential clinical use in the future. Moreover, from a methodological point of view this study increases knowledges on how vaccine route(s) can affect resulting immune responses and into mechanisms involved in the induction of humoral and cellular immunity to vaccine.
