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Characterization of a novel 3D-FN-silk breast cancer model

ABSTRACT: Characterization of a novel 3D-FN-silk breast cancer model

PROVIDER: PRJNA861264 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR20427540_1.fastq.gz	Fastqsanger.gz
	SRR20427540_2.fastq.gz	Fastqsanger.gz
	SRR20427541_1.fastq.gz	Fastqsanger.gz
	SRR20427541_2.fastq.gz	Fastqsanger.gz
	SRR20427542_1.fastq.gz	Fastqsanger.gz

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Characterization of a novel 3D-FN-silk breast cancer model

Project description:To offer a robust and highly characterized three-dimensional (3D) model for anti-cancer drug discovery and basic research, we developed a 3D system in which the immortalized breast cancer cell lines MCF-7 and MDA-MB-231 were grown in recombinantly produced spider silk functionalized with the cell adhesion motif from fibronectin (FN-silk). The aim of this study is to evaluate whole-transcriptome changes driven by growth in such 3D model.

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miRNA Nanostring Data on a Silk Scaffold model following CCI injury

Project description:This study investigates the effects of controlled cortical impact (CCI), a form of traumatic brain injury (TBI), using a 3D silk scaffold cell culture model. Human induced neural stem cells (iNSCs), human induced astrocytes, and HMC3 microglial cells were seeded onto the silk scaffolds and allowed to grow under controlled conditions. A subset of these cell cultures was then subjected to CCI to simulate injury. The primary aim of the study was to assess the cellular and molecular response to CCI, particularly focusing on miRNA expression profiles. miRNA Nanostring technology was employed to quantify differential expression patterns associated with CCI exposure, providing insights into neuroinflammatory, regenerative, and apoptotic pathways impacted by the injury. These data contribute to a better understanding of miRNA's role as a biomarker for TBI and its potential involvement in cellular recovery and neuroprotection processes following traumatic injury.

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High-throughput Sequencing of Small RNAs from Pollen and Silk and Characterization of miRNAs Involved in Pollen-Silk Interactions in Maize

Project description:In this study, we sequenced four small RNA libraries derived from mature pollens, in vitro germinated pollens, mature silks and pollinated silks of maize, respectively. In total, 161 known miRNAs belonging to 27 families and 82 novel miRNAs were identified. Of them, miRNAs involved in pollen-silk (pistil) interactions were analyzed. On the male side, miRNA differentially expressed between mature and germinated pollen were identified, some of them participate in pollen germination and tube growth. On the female side, silk-expressed miRNAs respond to pollination were also responsive to stresses, especially drought and fungal invasion. Furthermore, GO analysis of target genes revealed that members related to anxin signal transduction and gene expressional regulation were overrepresented.The results indicated that during pollen-silk interactions, miRNAs-mediated auxin signal transduction plays important roles, and miRNAs took part in complex transcriptional regulating network.

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