Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcript profile analyses of maize silk strands during silk senescence

ABSTRACT: To fully characterize the extent of genomic changes in senescent silk, we performed a time-course tissue harvesting. Material was collected at four time points: 3-, 7-, 11- and 15 days after silk emergence (DASE). Maize inbred line B104; 2 cm of basal part of silk from rings 6-10

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Zea mays

SUBMITTER: Maria Simaskova

PROVIDER: E-MTAB-10847 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Publications

KIL1 terminates fertility in maize by controlling silk senescence.

Šim Škov M Ria MR Daneva Anna A Doll Nicolas N Schilling Neeltje N Cubr A-Rad O Marta M Zhou Liangzi L De Winter Freya F Aesaert Stijn S De Rycke Riet R Pauwels Laurens L Dresselhaus Thomas T Brugi Re Norbert N Simmons Carl R CR Habben Jeffrey E JE Nowack Moritz K MK Nowack Moritz K MK

The Plant cell 20220701 8

Plant flowers have a functional life span during which pollination and fertilization occur to ensure seed and fruit development. Once flower senescence is initiated, the potential to set seed or fruit is irrevocably lost. In maize, silk strands are the elongated floral stigmas that emerge from the husk-enveloped inflorescence to intercept airborne pollen. Here we show that KIRA1-LIKE1 (KIL1), an ortholog of the Arabidopsis NAC (NAM (NO APICAL MERISTEM), ATAF1/2 (Arabidopsis thaliana Activation F ...[more]

PMID: 35608197

Similar Datasets

Project description:Spider silk proteins are synthesized in the silk-producing glands, where the spidroins are produced, stored and processed into a solid fiber from a crystalline liquid solution. Despite great interest in the spider silk properties, that make this material suitable for biomedical and biotechnological applications, the mechanism of formation and spinning of the silk fibers has not been fully elucidated; and no combination of proteomic and transcriptomic study has been carried out so far in the spider silk-producing glands. Nephila clavipes is an attractive orb-web spider to investigate the spinning process of silk production, given the properties of strength, elasticity and biocompatibility of their silk fibers. Thus, considering that the combination of proteomic and transcriptomic analysis may reveal an extensive repertoire of novel proteins involved in the silk spinning process, and in order to facilitate and enable proteomics in this non-model organism, the current study aims to construct a high quality reference mRNA-derived protein database that could be used to identify tissue specific expression patterns in spider silk glands. Next-generation sequencing has offered a powerful and cost-efficient technique for the generation of transcriptomic datasets in non-model species using diverse platforms such as the Illumina HiSeq, Roche 454, Pacific Biosystems, and Applied Biosystems SOLiD; In the current study, the Illumina HiSeq 2000 platform will be used to generate a N. clavipes spider silk glands transcriptome-based protein database. The transcriptome data generated in this study will provide a comprehensive and valuable genomic resource for future research of the group of spider silk-producing glands, in order to improve our understanding of the overall mechanism of action involved in production, secretion, storage, transport, protection and conformational changes of spidroins during the spinning process, and prey capture; and the results may be relevant for scientists in material Science, biology, biochemistry, and environmental scientists.

Dataset Information

Transcript profile analyses of maize silk strands during silk senescence

Publications

KIL1 terminates fertility in maize by controlling silk senescence.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets