Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Cytoglobin dependent transcriptomic changes determine sensitivity of melanoma to ferroptopsis

ABSTRACT: Cytoglobin dependent transcriptomic changes determine sensitivity of melanoma to ferroptopsis

PROVIDER: PRJNA862449 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR20649106_1.fastq.gz	Fastqsanger.gz
	SRR20649106_2.fastq.gz	Fastqsanger.gz
	SRR20649107_1.fastq.gz	Fastqsanger.gz
	SRR20649107_2.fastq.gz	Fastqsanger.gz
	SRR20649108_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

Cytoglobin dependent transcriptomic changes determine sensitivity of melanoma to ferroptopsis

Project description:In this study, we investigated the potential effect of CYGB on the cellular sensitivity towards (1S, 3R)-RAS-selective lethal small molecule (RSL3)-mediated ferroptosis in the G361 melanoma cells with abundant endogenous expression. Our findings show that an increased basal ROS level and higher degree of lipid peroxidation upon RSL3 treatment contributes to the increased sensitivity of CYGB knockdown G361 cells to ferroptosis. Furthermore, transcriptome analysis demonstrates the enrichment of multiple cancer malignancy pathways upon CYGB knockdown, supporting a tumor suppressive role for CYGB. Remarkably, CYGB knockdown also triggered activation of the NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3) inflammasome and subsequent induction of pyroptosis target genes. Altogether, we show that silencing of CYGB expression modulates cancer therapy sensitivity via modulation of ferroptosis and pyroptosis cell death signalling pathways.

2022-07-29 | GSE209775 | GEO

Cytoglobin overexpression-dependent transcriptomic changes in A375 melanoma cells

Project description:Cytoglobin overexpression-dependent transcriptomic changes in A375 melanoma cells

| PRJNA1010179 | ENA

Cytoglobin overexpression-dependent transcriptomic changes in A375 melanoma cells

Project description:Cytoglobin (CYGB) is a member of the oxygen-binding globin superfamily. In this study, we comprehensively explored the CYGB-dependent transcriptome in A375 melanoma cells overexpressing CYGB. Our findings reveal that CYGB overexpression positively enriches cancer-associated pathways, including the mTORC1 and AKT/mTOR signaling pathways, which are frequently overactivated in tumors. Moreover, several cancer-associated pathways, such as epithelial-mesenchymal transition (EMT) mediated by CSPG4, were downregulated upon CYGB overexpression. Intriguingly, our results indicate that CYGB overexpression leads to a reduction in the inflammatory state of melanoma cells. This anti-inflammatory potential is exemplified by the downregulation of key inflammasome-associated genes, including NLRP1, CASP1, and CD74, which play pivotal roles in cytokine regulation and inflammasome activation. Consistent with established globin functions, CYGB appears to play a crucial role in redox homeostasis. Furthermore, our study highlights CYGB's involvement in DNA repair mechanisms and its regulation of NOX4, reinforcing its versatility in safeguarding genomic integrity. Collectively, our data illuminate the diverse functions of CYGB in melanoma cells, pointing to its roles in cellular protection against oxidative stress, inflammation, and cancer-associated pathways. These findings pave the way for further research into the physiological role of CYGB and its potential as a therapeutic target in melanoma.

2024-08-21 | GSE241816 | GEO

Developmental chromatin programs determine oncogenic competence in melanoma

Project description:Developmental chromatin programs determine oncogenic competence in melanoma

| PRJNA721948 | ENA

Developmental chromatin programs determine oncogenic competence in melanoma [Zebrafish]

Project description:Developmental chromatin programs determine oncogenic competence in melanoma [Zebrafish]

| PRJNA721737 | ENA

Harnessing BET inhibitor sensitivity reveals AMIGO2 as a melanoma survival gene.

Project description:We utilize the transcriptional effects of BETi in melanoma and identify AMIGO2 as a direct target gene essential for melanoma cell survival both in vitro and in vivo. We further map the enhancer landscape of NHM and melanooma and show that genes regulated by super enhancers are expressed in higher levels, exihibit higher sensitivity to BETi, and over expressed in melanoma relative to NHM. In melanoma, AMIGO2 is regulated by super enhancers, which upon BETi lose their BRD2/BRD4 enrichment, resulting in AMIGO2 silencing.

2017-11-16 | GSE94488 | GEO

Histone variant H2A.Z.2 mediates proliferation and drug sensitivity of malignant melanoma (expression)

Project description:Here we report a novel role for H2A.Z.2 (H2AFV) as a mediator of cell proliferation and sensitivity to targeted therapies in malignant melanoma. While both H2A.Z.1 and H2A.Z.2 are highly expressed in metastatic melanoma and correlate with decreased patient survival, only H2A.Z.2 deficiency results in impaired cellular proliferation of melanoma cells, which occurs via a G1/S arrest. Integrated gene expression and ChIP-seq analyses revealed that H2A.Z.2 positively regulates E2F target genes, and that such genes acquire a distinct H2A.Z occupancy signature over the promoter and gene body in metastatic melanoma cells. We further identified the BET family member BRD2 as an H2A.Z-interacting protein in melanoma cells, and demonstrate that H2A.Z.2 silencing cooperates with BET inhibition to induce cell death. Total RNA samples from SKmel147 melanoma cell line. Transcript levels were analyzed after control or H2AZ variant RNA interference.

2015-07-01 | E-GEOD-59026 | biostudies-arrayexpress

Inhibition of NF-κB-dependent signaling enhances sensitivity and overcomes resistance to BET inhibition in uveal melanoma

Project description:Inhibition of NF-κB-dependent signaling enhances sensitivity and overcomes resistance to BET inhibition in uveal melanoma

| PRJNA510635 | ENA

Developmental chromatin programs determine oncogenic competence in melanoma [Cut&Run]

Project description:Developmental chromatin programs determine oncogenic competence in melanoma [Cut&Run]

| PRJNA721947 | ENA

Developmental chromatin programs determine oncogenic competence in melanoma [RNA-seq]

Project description:Developmental chromatin programs determine oncogenic competence in melanoma [RNA-seq]

| PRJNA721733 | ENA