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Cytoglobin overexpression-dependent transcriptomic changes in A375 melanoma cells

ABSTRACT: Cytoglobin overexpression-dependent transcriptomic changes in A375 melanoma cells

PROVIDER: PRJNA1010179 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25779131_1.fastq.gz	Fastqsanger.gz
	SRR25779131_2.fastq.gz	Fastqsanger.gz
	SRR25779132_1.fastq.gz	Fastqsanger.gz
	SRR25779132_2.fastq.gz	Fastqsanger.gz
	SRR25779133_1.fastq.gz	Fastqsanger.gz

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Cytoglobin overexpression-dependent transcriptomic changes in A375 melanoma cells

Project description:Cytoglobin (CYGB) is a member of the oxygen-binding globin superfamily. In this study, we comprehensively explored the CYGB-dependent transcriptome in A375 melanoma cells overexpressing CYGB. Our findings reveal that CYGB overexpression positively enriches cancer-associated pathways, including the mTORC1 and AKT/mTOR signaling pathways, which are frequently overactivated in tumors. Moreover, several cancer-associated pathways, such as epithelial-mesenchymal transition (EMT) mediated by CSPG4, were downregulated upon CYGB overexpression. Intriguingly, our results indicate that CYGB overexpression leads to a reduction in the inflammatory state of melanoma cells. This anti-inflammatory potential is exemplified by the downregulation of key inflammasome-associated genes, including NLRP1, CASP1, and CD74, which play pivotal roles in cytokine regulation and inflammasome activation. Consistent with established globin functions, CYGB appears to play a crucial role in redox homeostasis. Furthermore, our study highlights CYGB's involvement in DNA repair mechanisms and its regulation of NOX4, reinforcing its versatility in safeguarding genomic integrity. Collectively, our data illuminate the diverse functions of CYGB in melanoma cells, pointing to its roles in cellular protection against oxidative stress, inflammation, and cancer-associated pathways. These findings pave the way for further research into the physiological role of CYGB and its potential as a therapeutic target in melanoma.

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Analysis of gene expression profiles of human melanoma A375 cells with or without CD10 overexpression

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Effects of mcFA on epigentic changes in A375 melanoma [ATAC-seq]

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Project description:Identify transcriptionnally and translationally regulated mRNA in melanoma parental and persister cells In this dataset, we include expression data of A375 melanoma drug-naïve parental cells and A375 melanoma persister cells that survived from BRAF and MEK inhibition. The expression data are studied in both total RNA and polysome-bounded RNA.

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Effects of mcFA on epigentic changes in A375 melanoma. [ChIP-seq]

Project description:To study the epigenome profile of melanoma cells upon mcFAs administration, we stimulated A375 cells using octanoate for 48-hrs followed by H3K27ac ChIP-seq analysis with 2 biological replicates.

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A375 melanoma cells treated with leflunomide 25uM

Project description:Human A375 melanoma cells were treated with leflunomide 25uM for 3 days, then RNA harvested 6 arrays

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A375 melanoma cells treated with leflunomide 25uM

Project description:Human A375 melanoma cells were treated with leflunomide 25uM for 3 days, then RNA harvested

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Expression data from catalase stably transfected A375 human melanoma cells

Project description:Reactive oxygen species (ROS) are implicated in tumor transformation by modulating proteins involved in differentiation, proliferation and invasion. In order to identify genes that may support melanoma progression or regression after an antioxidant system (AOS) response, we developed and characterized a human melanoma cell model with different levels of ROS by stably overexpressing the antioxidant enzyme catalase in A375 amelanotic melanoma cells, and whole genome gene expression patterns were analyzed by microarrays. We used gene expression microarrays to study the AOS global response to catalase overexpression and to identify up-regulated and down-regulated genes during progression or regression of melanoma.

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