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Effects of adipocyte-conditioned medium on triple-negative breast cancer cells

ABSTRACT: Effects of adipocyte-conditioned medium on triple-negative breast cancer cells

PROVIDER: PRJNA862897 | ENA |

REPOSITORIES: ENA

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Effects of adipocyte-conditioned medium on triple-negative breast cancer cells

Project description:The disturbance of adipose tissue in obesity highly correlates with cancer progression. To investigate the impacts of obesity on triple-negative breast cancer (TNBC), we performed an in vitro system culturing TNBC cells with human white adipocytes-conditioned medium (hAd-CM). The gene expression profiling between each condition was then assessed by RNA-seq.

2023-01-25 | GSE209894 | GEO

Macrophage reponse to triple-negative breast cancer cells in vitro

Project description:Gene expression profiling of monocyte-derived macrophage polarised by triple-negative breast cancer cell conditioned medium

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Analysis by Whole Transcriptome Sequencing of the Effects of LLC1 conditioned medium, LLC1 conditioned plus Calcitriol, non-conditioned medium, and non-conditioned medium plus Calcitriol on mRNA Expression in Primary Human Skeletal Muscle Cells

Project description:Primary human skeletal muscle cells (Lonza) were treated with LLC1 conditioned medium, LLC1 conditioned medium plus Calcitriol, LLC1 non-conditioned medium or LLC1 non-conditioned medium plus Calcitriol for a period of 24 hours prior to isolation of RNA.

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RNA from mouse lung conditioned medium

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Human Adipocytes Regulate Gene Expression in Triple-negative Breast Cancer Assessed by NGS Sequencing

Project description:Adipocyte was found play a pivotal role in tumorgenesis, progression and metastasis in breast cancer. However, affection of adipocyte on gene expression profile in triple-negative breast cancer (TNBC) is still not clear. In the present study, firstly we reported the gene expression profiles of TNBC regulated by human adipocytes using NGS. TNBC cell MDA-MB-231 was used in this study. MDA-MB-231 cells were treated with medium derived from adipocytes culture supernatants. Using two different RNA libraries, we sequenced the complete transcriptome(including mRNA, lncRNA, circleRNA and small RNA) of MDA-MB-231 treated with medium derived from adipocytes culture supernatants.

2018-09-04 | GSE119377 | GEO

Metabolomics of Adipocyte-Conditioned Media Compared to Stromal Cell- and Un-conditioned Media

Project description:The metabolomics profiles of adipocyte conditioned media (ACM), stromal cell conditioned media (SCM) and unconditioned media (UCM) were analyzed by untargeted mass spectrometry.

2022-10-21 | ST002399 | MetabolomicsWorkbench

Protein Amount in Conditioned Medium of MenSCs

Project description:Protein Amount in Conditioned Medium of MenSCs

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RNA from mouse lung conditioned medium

Project description:RNA fragments were isolated from mouse lung conditioned medium and analyzed by a microarray This analysis surveys RNAs, probably complexed with RNA-binding protein, secreted in the culture media

2021-04-11 | GSE161219 | GEO

RNA-seq of murine macrophages (J774A.1 and Raw264.7) grown for 48 hours in conditioned media collected from primary murine Triple Negative Breast Cancer cells

Project description:The goal of the experiment was to demonstrate if the overexpression of human-Prune-1 in Triple Negative breast cancer cells induces M2-polarization of macrophages in vitro. For this purpose, murine primary cells from breast tumor developed by Genetically Engineered Mouse Models (GEMMs) of TNBC (i.e., MMTV-Wnt1) and metastatic TNBC overexpressing both human Prune-1 and Wnt1 in mammary gland (i.e., MMTV-Prune-1/Wnt1) were obtained. Conditioned media were collected from these primary cells (1x106 cells) after 24 hours. Murine macrophages (J774A.1 and Raw264.7; 1x106) were starved for six hours and then grown for 48 hours in those conditioned media collected from MMTV-Wnt1 and MMTV-Prune-1/Wnt1 cells. Untreated macrophages were used as negative control for the experiment.

2020-12-03 | E-MTAB-9231 | biostudies-arrayexpress

RNA-seq data of human T cells treated with decidual cell conditioned medium

Project description:RNA-seq data of Naïve T cells treated with decidual cell conditioned medium. Naïve T cells were differentiated into Th17 cells by IL6, TGFB1, and IL23 stimulation, in the presence of either unconditioned (control) or conditioned medium from uterine decidual stromal cells

2020-07-01 | GSE120380 | GEO

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