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Effects of adipocyte-conditioned medium on triple-negative breast cancer cells

ABSTRACT: Effects of adipocyte-conditioned medium on triple-negative breast cancer cells

PROVIDER: PRJNA862897 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR20670481_1.fastq.gz	Fastqsanger.gz
	SRR20670481_2.fastq.gz	Fastqsanger.gz
	SRR20670483_1.fastq.gz	Fastqsanger.gz
	SRR20670483_2.fastq.gz	Fastqsanger.gz
	SRR20670485_1.fastq.gz	Fastqsanger.gz

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Effects of adipocyte-conditioned medium on triple-negative breast cancer cells

Project description:The disturbance of adipose tissue in obesity highly correlates with cancer progression. To investigate the impacts of obesity on triple-negative breast cancer (TNBC), we performed an in vitro system culturing TNBC cells with human white adipocytes-conditioned medium (hAd-CM). The gene expression profiling between each condition was then assessed by RNA-seq.

2023-01-25 | GSE209894 | GEO

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Project description:Adipocyte was found play a pivotal role in tumorgenesis, progression and metastasis in breast cancer. However, affection of adipocyte on gene expression profile in triple-negative breast cancer (TNBC) is still not clear. In the present study, firstly we reported the gene expression profiles of TNBC regulated by human adipocytes using NGS. TNBC cell MDA-MB-231 was used in this study. MDA-MB-231 cells were treated with medium derived from adipocytes culture supernatants. Using two different RNA libraries, we sequenced the complete transcriptome(including mRNA, lncRNA, circleRNA and small RNA) of MDA-MB-231 treated with medium derived from adipocytes culture supernatants.

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Project description:The metabolomics profiles of adipocyte conditioned media (ACM), stromal cell conditioned media (SCM) and unconditioned media (UCM) were analyzed by untargeted mass spectrometry.

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RNA from mouse lung conditioned medium

Project description:RNA fragments were isolated from mouse lung conditioned medium and analyzed by a microarray This analysis surveys RNAs, probably complexed with RNA-binding protein, secreted in the culture media

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RNA-seq of murine macrophages (J774A.1 and Raw264.7) grown for 48 hours in conditioned media collected from primary murine Triple Negative Breast Cancer cells

Project description:The goal of the experiment was to demonstrate if the overexpression of human-Prune-1 in Triple Negative breast cancer cells induces M2-polarization of macrophages in vitro. For this purpose, murine primary cells from breast tumor developed by Genetically Engineered Mouse Models (GEMMs) of TNBC (i.e., MMTV-Wnt1) and metastatic TNBC overexpressing both human Prune-1 and Wnt1 in mammary gland (i.e., MMTV-Prune-1/Wnt1) were obtained. Conditioned media were collected from these primary cells (1x106 cells) after 24 hours. Murine macrophages (J774A.1 and Raw264.7; 1x106) were starved for six hours and then grown for 48 hours in those conditioned media collected from MMTV-Wnt1 and MMTV-Prune-1/Wnt1 cells. Untreated macrophages were used as negative control for the experiment.

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Project description:Adipocyte-secreted factors (ASFs) induce lipid accumulation in breast cancer cells. These lipid droplets are thought to serve a cell-protective, pro-oncogenic role. ASFs including insulin, CCL5, and apelin interact with receptors that stimulate mTOR signaling in neighboring cells. One of the major transcription factors that acts downstream of this signaling pathway is SREBP1, which translocates into the nucleus and activates the expression of metabolic enzymes and other proteins involved in fatty acid synthesis, transport, and storage. Their associated enzymatic activities increase or decrease pools of metabolites that serve as substrates and cofactors for chromatin remodeling, such as acetyl-CoA, alpha-ketoglutarate (α-KG), flavin adenine dinucleotide (FAD+), and nicotinamide adenine dinucleotide (NAD+), but the impact on gene regulation in this context is not completely understood. To model biochemical signaling from adipocytes to breast cancer epithelial cells, we cultured differentiated mouse OP-9 cells, collected the adipocyte conditioned medium (ACM) after three days, and treated human breast epithelial BT-549 cells with ACM. Three days after treatment, we observed accumulation of lipid droplets in the cytoplasm of most BT-549 cells, whereas none were visible in cells grown in unconditioned medium (UCM). Transcription profiling verified the activation of lipid droplet biosynthesis genes, and revealed repression of loci at every chromosome, consistent with the idea that lipogenesis affects the availability of substrates and cofactors for global chromatin remodeling.

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