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Effects of adipocyte-conditioned medium on triple-negative breast cancer cells

ABSTRACT: The disturbance of adipose tissue in obesity highly correlates with cancer progression. To investigate the impacts of obesity on triple-negative breast cancer (TNBC), we performed an in vitro system culturing TNBC cells with human white adipocytes-conditioned medium (hAd-CM). The gene expression profiling between each condition was then assessed by RNA-seq.

ORGANISM(S): Homo sapiens

PROVIDER: GSE209894 | GEO | 2023/01/25

REPOSITORIES: GEO

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Effects of adipocyte-conditioned medium on triple-negative breast cancer cells

Project description:Effects of adipocyte-conditioned medium on triple-negative breast cancer cells

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Effect of exosomal miRNA of cancer-associated adipocyte (CAA) on the malignant transformation of pancreatic ductal adenocarcinoma

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2022-11-01 | PXD034550 | JPOST Repository

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RNA-seq of murine macrophages (J774A.1 and Raw264.7) grown for 48 hours in conditioned media collected from primary murine Triple Negative Breast Cancer cells

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LEP-MCH conditioned childhood obesity

Project description:LEP-MCH conditioned childhood obesity

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Proteomic analysis of extracellular vesicles and conditioned medium from human Adipose-derived Stem/Stromal Cells and Dermal Fibroblasts

Project description:Conditioned medium (CM) and extracellular vesicles (EV) from human Adipose-derived Stem/stromal cells (ASC) and Dermal fibroblasts (DF) can represent valid cell substitutes in regenerative medicine and other therapeutic applications. Whether one of the two cell products should be preferred over the other is still under debate and no direct comparison of their proteome has been reported yet. Here, we apply a comprehensive quantitative proteomics approach to explore the protein composition of conditioned medium and EV obtained from the two cell types. We identified 1977 proteins by LC-MS/MS proteomic analysis. Unsupervised clustering analysis and PCA clearly distinguished CM and EV as separate groups, regardless the cell source. We identified 68 and 201 proteins that were more abundant or exclusively detectable in CM and EV, respectively. CM were enriched in proteins of endoplasmic reticulum, Golgi apparatus and lysosomes, whereas EV contained a large amount of GTPases, ribosome and translation-related factors. Moreover, the analysis of ASC and DF secretomes (both CM and EV) revealed the presence of cell type-specific factors. ASC-CM and -EV carried factors involved in ECM organization (hyaluronan and glycosaminoglycan metabolism) and immunological regulation (e.g. macrophage and IkB/NFkB signaling regulation), respectively. On the other hand, DF-CM and –EV were both enriched in epithelium development associated factors, whilst DF-CM in proteins involved in cellular processes regulation and -EV in Wnt signaling factors. In conclusion, this comprehensive proteomic analysis of the secretome components of ASC and DF, provides evidence of a different protein composition between CM and EV and of the presence of cell type-specific bioactive mediators suggesting their specific future use as advanced therapy medicinal products.

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