Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A BNC1-IRF6-AP1 transcriptional network in keratinocytes and Squamous Cell Carcinoma [ChIP-Seq]

ABSTRACT: A BNC1-IRF6-AP1 transcriptional network in keratinocytes and Squamous Cell Carcinoma [ChIP-Seq]

PROVIDER: PRJNA863285 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR20695592.fastq.gz	Fastqsanger.gz
	SRR20695593.fastq.gz	Fastqsanger.gz
	SRR20695594.fastq.gz	Fastqsanger.gz
	SRR20695595.fastq.gz	Fastqsanger.gz
	SRR20695596.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

A BNC1-IRF6-AP1 transcriptional network in keratinocytes and Squamous Cell Carcinoma [RNA-Seq]

Project description:Analysis of transcriptional regulation by BNC1, IRF6, and FRA1 in keratinocyes and Squamous Cell Carcinoma cell lines

2024-12-31 | GSE212905 | GEO

A BNC1-IRF6-AP1 transcriptional network in keratinocytes and Squamous Cell Carcinoma [ChIP-Seq]

Project description:Analysis of BNC1, IRF6, FRA1, and JUNB genome binding/occupancy in epidermal keratinocyes and cutaneous Squamous Cell Carcinoma cell lines

2024-12-31 | GSE209993 | GEO

A BNC1-IRF6-AP1 transcriptional network in keratinocytes and Squamous Cell Carcinoma

Project description:A BNC1-IRF6-AP1 transcriptional network in keratinocytes and Squamous Cell Carcinoma

| PRJNA878091 | ENA

A BNC1-IRF6-AP1 transcriptional network in keratinocytes and Squamous Cell Carcinoma [RNA-Seq]

Project description:A BNC1-IRF6-AP1 transcriptional network in keratinocytes and Squamous Cell Carcinoma [RNA-Seq]

| PRJNA878174 | ENA

Interferon Regulatory Factor 6 (IRF6) knockout in human keratinocytes disrupts epithelial homeostasis

Project description:The transcription factor Interferon Regulatory Factor 6 (IRF6) is crucially involved in craniofacial development and regulates the proliferation/differentiation balance in keratinocytes. Pathological IRF6 variants have been found in Van der Woude syndrome (VWS), the most common syndromic form of cleft lip / palate (CLP) as well as in non-syndromic CLP cases. Besides its prominent function in regulating keratinocyte differentiation, recent data revealed that IRF6 is also involved in wound healing and migration. Since a significant fraction of CLP patients undergoing corrective cleft surgery experience wound healing complications, IRF6 represents an interesting candidate gene linking the two processes. However, Irf6 function has been mainly studied in mice and knowledge on IRF6 in human cells remains sparse. Here, we aimed to elucidate the role of IRF6 in human postnatal skin- and oral mucosa-derived keratinocytes by its ablation using CRISPR/Cas9. We complement this approach by applying proteomics and identify that lack of IRF6 disrupts human epithelial homeostasis by altering cell colony morphology, migration pattern, and the differentiation potential of keratinocytes.

2022-02-17 | PXD027669 | Pride

A BNC1-IRF6-AP1 transcriptional network in keratinocytes and Squamous Cell Carcinoma

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-12-31 | GSE212906 | GEO

RNAseq E15.5 mouse skin WT, Irf6 KO, Ripk4 KD, and Irf6 S413A.S424A knockin

Project description:The integrity of the mammalian epidermis is essential for organism survival, and it depends on a balance of proliferation and differentiation in the resident stem cell population. The kinase Ripk4 and the transcription factor Irf6 are mutated in severe developmental syndromes in humans, and mice lacking these genes display epidermal hyperproliferation and soft tissue fusions, resulting in neonatal lethality. However, the mechanism by which these genes control epidermal differentiation in vivo is unknown. By generating various mouse knock-out and knock-in strains we demonstrate that in vivo the role of Ripk4 in development is dependent on its kinase activity, Ripk4 and Irf6 function cell autonomously in the epidermis,Ripk4 and Irf6 lie on a linear pathway and phosphorylation of Irf6 on Serine413 and Serine424 is essential to prime it for activation. This priming then allows Ripk4 to phosphorylate Irf6 on Serine90, which ensures Irf6 activation. We then use RNA-seq, ChIP-seq and ATAC-seq analysis to define the global transcriptional targets of Irf6 in epidermal differentiation. Collectively, our results explain how Ripk4 activates Irf6, and how this pathway ensures epidermal differentiation and a functional barrier. This is crucial for understanding the etiology of developmental syndromes that are characterized by orofacial, skin and genital abnormalities.

2019-08-29 | GSE124047 | GEO

AP1

Project description:Arabidopsis thaliana AP1, AP1 [Source:UniProtKB/TrEMBL;Acc:A0A178W846], is differentially expressed in 21 experiment(s);

| AT1G69120 | ExpressionAtlas

AP1

Project description:Arabidopsis thaliana AP1, AP1 [Source:UniProtKB/TrEMBL;Acc:A0A178W846], is expressed in 8 baseline experiment(s);

| AT1G69120 | ExpressionAtlas

Irf6 wildtype and knock out ATAC-seq

2019-08-29 | GSE127341 | GEO