Project description:Differentially expressed genes were identified by comparing the gene expression profiling of RAW264.7 of Ep300 silencing with that of control. Results provide important information to indicate Effect of Ep300 silencing on RAW264.7.
Project description:Mycobacterial transcripts were identified in exosomes released from M.tb infected RAW264.7 macrophages that were not present in uninfected exosomes suggesting export of mycobacterial RNA via exosomes Mycobacterial RNA was used as positive control and RNA from exosomes released from uninfected macrophages was used as negative control
Project description:In sepsis, infection and immunosuppression occur simultaneously. We currently found that Samsn1 expression is increased on myeloid cells in sepsis. And, Samsn1 is currently recognized as a negative regulator of immune response. So we constructed Samsn1 knockout Raw264.7 cells to study the effect of Samsn1 on macrophage function and the gene expression of macrophages with infection.
Project description:Gene expression profile of FABP4 treatment in RAW264.7 macrophages was examined to show a ligand (palmitic acid)-dependent and a ligand-independent effect of FABP4. RAW264.7 macrophages were treated with and without 200 nM recombinant FABP4 in the absence and presence of 0.2 mM palmitic acid.
Project description:To study the effect of iodide ions on the M1 polarization of macrophages, we chose the RAW264.7 cell line. We then performed gene expression profiling analysis using data obtained from RNA-seq of 3 different groups.