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Gene expression profiles of Wnt4 high and Wnt4 low populations of murine islets at postnatal day 1 (P1).

ABSTRACT: Gene expression profiles of Wnt4 high and Wnt4 low populations of murine islets at postnatal day 1 (P1).

PROVIDER: PRJNA864809 | ENA |

REPOSITORIES: ENA

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Gene expression profiles of Wnt4 high and Wnt4 low populations of murine islets at postnatal day 1 (P1).

Project description:To find the differences between β cells expressing Wnt4 and those that do not express it (or express it at low levels) at neonatal stage, murine islets at postnatal day 1 (P1) were collected. Based on a reporter expressing Cre recombinase from the Wnt4 locus, we observed that Wnt4 expression starts in a small subset of β cells at embryonic day (E) 18.5. At neonatal stages, around 50% of β cells express Wnt4 at P0 and most of cells express it by P5. We collected Wnt4 high and Wnt4 low islet cells by FACS and compared their gene expression profile. Wnt4 high islet cell population showed a significant increase of β-cell maturation and function related genes, including transcription factors such as Pdx1 and Nkx6.1, insulin 2, glucokinase (Gck), and the glucose transporter Slc2a2. On the contrary, Wnt4 low islet cell population showed significant increasing of proliferation markers such as Pcna, Mki67 and the positive cell cycle regulators Ccnd1, Ccnd3, Ccna2, Cdk1, Cdk2 and Cdk6. Taken together, these results show that Wnt4 expression is higher in more mature but less proliferative β cells.

2022-08-02 | GSE210237 | GEO

Gene expression profiles of islets of Langerhans isolated from 7 weeks wt and Wnt4 conditional knock outs.

Project description:To check the role of Wnt4 in murine β cells function, we inactivated Wnt4 in β cells from 4-5 weeks using tamoxifen. After 2 weeks tamoxifen treatment, we collected islets from control and mutant mice. We found that Wnt4 knock out islets show significant reduction of oxidation-reduction processes, cysteine and methionine metabolism, fatty acid beta-oxidation and fatty acid biosynthesis, as well as transport mechanisms. On the contrary, ribosome related genes, as well as oxidative stress and apoptosis genes were up-regulated.

2022-08-04 | GSE210267 | GEO

Effects of Adjudin on gene expression of postnatal day 0 mouse pancreatic islets

Project description:To investigate effects of Adjudin on gene expression of postnatal day 0 mouse islets (P0 islets), islets from postnatal day 0 mouse (regardless of sex) were isolated, cultured in incubator for overnight recovery, and treated with either DMSO or 10 µM Adjudin for 1 day before RNA sequencing.

2023-07-07 | GSE235397 | GEO

Effect of IUGR on postnatal day 14 pancreatic islets

Project description:IUGR is a common complication of pregnancy. We have created a rat model of IUGR which mimics placental insufficiency. Surgery is performed at embryonic day 18 and dams are allowed to deleiver spontaneously. At postnatal day 14, we isolate pancreatic islets and have compared their gene expression with that of control (sham) surgery animals. Two-condition experiment, control vs IUGR pancreatic islets. Biological replicates: 4 control replicates, 4 IUGR replicates.

2013-07-13 | E-GEOD-48822 | biostudies-arrayexpress

Effect of IUGR on postnatal day 14 pancreatic islets

2013-07-13 | GSE48822 | GEO

Single-cell RNA sequencing (scRNAseq) data from postnatal day (P) 5 Nes-Cfp/+ cerebella from Wild type (non-irradiated) and Irradiated (at P1) pups

Project description:We performed large-scale single cell profiling of dissociated whole postnatal day (P) 5 cerebella from 4 male NestinCFP/+ pups that were either irradiated at P1, or their control littermates in two replicate experiments.

2021-12-08 | GSE173091 | GEO

WNT4 is required for ovarian follicle development and female fertility

Project description:To study the physiological role of WNT4 in the postnatal ovary, a mouse strain bearing a floxed Wnt4 allele was created and mated to the Amhr2tm3(cre)Bhr strain to target deletion of Wnt4 to granulosa cells. Wnt4flox/-;Amhr2tm3(cre)Bhr/+ mice had significantly reduced ovary weights and produced smaller litters (P<0.05). Serial follicle counting demonstrated that, while Wnt4flox/-;Amhr2tm3(cre)Bhr/+ mice were born with a normal ovarian reserve and maintained normal numbers of small follicles until puberty, they had only 25.2% of the normal number of healthy antral follicles. Some Wnt4flox/-;Amhr2tm3(cre)Bhr/+ mice had no antral follicles or corpora lutea and underwent premature follicle depletion. RTPCR analyses of Wnt4flox/-;Amhr2tm3(cre)Bhr/+ granulosa cells and cultured granulosa cells that overexpress WNT4 demonstrated that WNT4 regulates the expression of Star, Cyp11a1 and Cyp19, steroidogenic genes previously identified as downstream targets of the WNT signaling effector CTNNB1. WNT4- and CTNNB1-overexpressing cultured granulosa cells were analyzed by microarray for alterations in gene expression, which showed that WNT4 also regulates a series of genes involved in late follicle development and the cellular stress response via the WNT/CTNNB1 signaling pathway. Together, these data indicate that WNT4 is required for normal antral follicle development, and may act by regulating granulosa cell functions including steroidogenesis.

2009-10-23 | GSE18704 | GEO

Effects of Adjudin on gene expression of postnatal day 0 mouse pancreatic islets

Project description:Effects of Adjudin on gene expression of postnatal day 0 mouse pancreatic islets

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SNAT - Single cell RNA sequencing of mouse sorted Schwann cells at postnatal day 1 (P1)

Project description:SNAT - Single cell RNA sequencing of mouse sorted Schwann cells at postnatal day 1 (P1)

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SNAT - Single cell RNA sequencing of mouse cells sorted from sciatic nerves at postnatal day 1 (P1) and P60

Project description:Sciatic nerves from P1 and from P60 mice were dissociated, and the resulting cell suspension was sorted with FACS. All cells positive for beta-actin dsred (including Schwann cells also expressing eYFP due to P0Cre-mediated recombination) were collected for RNA sequencing with 10X Genomics.

2021-04-22 | GSE138577 | GEO

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