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ABSTRACT: Transcriptome sequencing of olive flounder (Paralichthys olivaceus)

PROVIDER: PRJNA867739 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR20994464.fastq.gz	Fastqsanger.gz
	SRR20994465.fastq.gz	Fastqsanger.gz
	SRR20994466.fastq.gz	Fastqsanger.gz
	SRR20994467.fastq.gz	Fastqsanger.gz
	SRR20994468.fastq.gz	Fastqsanger.gz

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MicroRNA map of Paralichthys olivaceus spleen at 6h and 24h after Streptococcus iniae infection

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Project description:In order to identify the genes induced by different bacterial cells, which may contain different types of pathogen associated molecular patterns (PAMPs), high-throughput gene expression analyses using Agilent custom-oligo DNA microarray containingon 9,573 probes constructed for Japanese flounder Paralichthys olivaceus were conducted. A number of genes showed significant changes in mRNA levels. However, there are no significant difference in a manner of the changes among the different bacterial cell treatments. The genes significantly induced by the treatments included well-known immune-related genes such as granulocyte-colony stimulation factor, haptoglobin, hepcidin. The kidney were isolated from the formalin killed cells (FKCs) intraperitoneal injected Japanese flounder, Paralichthys olivaceus using the four formalin-killed cells, Edwardsiella tarda strain 54, Lactococcus garviae strain EH8706, Streptococcus iniae strain 02 and Vibrio anguillarum strain H775-3, respectively. Fishes were administered by an intraperitoneal injection using the 1.0 x 10^7 to 1.0 x 10^8 cells of FKCs. After 6 hours from a injection, fish kidney was isolated. We also isolated phosphate-bufferd seline injected fish kidney as a control. We analyzed a four samples in control. We also analyze a three samples in FKC injected fish (16 hybridization).

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Gene expression profiles of vaccinated fish using a long-term efficiency mixed vaccine against Edwardsiella tarda performed by a new designed oligo DNA microarray in Japanese flounder Paralichthys olivaceus

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