Fish gut microbiome
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA868917 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Rainbow trout gut microbiota influenced by dietary black soldier fly larvae
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Project description:Time-course transcriptomic profilling of the oleaginous yeast Yarrowia lipolytica, during a controlled fed-batch. A nitrogen limitation was applied during the course of the fed-batch to initiate de novo biolipid synthesis.
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Project description:Early life gut microbiota plays a critical role in gut development, maturation of the immune response and protection against enteric pathogens such as Salmonella. In this study, we investigated how different gut microbiota compositions influence the host transcriptomic signatures and susceptibility to Salmonella infection in chicks.
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Project description:Gut microbiota dysbiosis characterizes systemic metabolic alteration, yet its causality is debated. To address this issue, we transplanted antibiotic-free conventional wild-type mice with either dysbiotic (“obese”) or eubiotic (“lean”) gut microbiota and fed them either a NC or a 72%HFD. We report that, on NC, obese gut microbiota transplantation reduces hepatic gluconeogenesis with decreased hepatic PEPCK activity, compared to non-transplanted mice. Of note, this phenotype is blunted in conventional NOD2KO mice. By contrast, lean microbiota transplantation did not affect hepatic gluconeogenesis. In addition, obese microbiota transplantation changed both gut microbiota and microbiome of recipient mice. Interestingly, hepatic gluconeogenesis, PEPCK and G6Pase activity were reduced even once mice transplanted with the obese gut microbiota were fed a 72%HFD, together with reduced fed glycaemia and adiposity compared to non-transplanted mice. Notably, changes in gut microbiota and microbiome induced by the transplantation were still detectable on 72%HFD. Finally, we report that obese gut microbiota transplantation may impact on hepatic metabolism and even prevent HFD-increased hepatic gluconeogenesis. Our findings may provide a new vision of gut microbiota dysbiosis, useful for a better understanding of the aetiology of metabolic diseases. all livers are from NC-fed mice only.
2017-03-16 | GSE81318 | GEO
Project description:Dietary supplementation of yeast Yarrowia lipolytica in rainbow trout (Oncorhynchus mykiss): Modulation of gut microbiota, mucosal health and immunity
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Project description:Gut function exhibits 24h (circadian) rhythmicity, in part driven by intrinsic clocks within intestinal epithelial cells (IECs). The gut microbiome exhibits 24h rhythms in composition and function, which are important for maintenance of metabolic and immune health. We determined the influence of feeding behaviour on the colonic circadian landscape using an interval feeding paradigm, whereby food intake was partitioned equally across the 24h day. RNAseq analysis revealed that the IEC cell intrinsic clock persists in the absence of diurnal feeding rhythms, however a subset of transcripts lose rhythmicity, demonstrating that feeding driven cell extrinsic temporal cues contribute significantly to maintenance of the rhythmic gut transcriptome. Interval fed mice lost rhythmicity in secretory IgA and within the microbiota and microbial derived short chain fatty acids. This work highlights the importance of daily rhythms in feeding behaviour for maintenance of rhythmic processes within the gut, with implications for metabolic and immune health.
2025-11-20 | E-MTAB-14883 | biostudies-arrayexpress
Project description:Total RNA versus genomic DNA hybridization on custom arrays designed for all Yarrowia lipolytica genes Total RNA was collected in mid-log phase from Yarrowia lipolytica cells grown in rich medium (abbreviated CM, in house recipe). RNA was then converted to cDNA, Cy3-labeled and hybridized competitively against Cy5 labeled genomic DNA from Yarrowia lipolytica.
2010-07-06 | E-GEOD-22192 | biostudies-arrayexpress
Project description:Investigation of whole genome gene expression level changes in a Yarrowia lipolytica Y4184 snf1 mutant, compared to the Y4184U+. The Y4184 is an engineered strain to produce eicosapentaenoic acid (EPA) via expression of a M-NM-^T9 elongase/M-NM-^T8 desaturase pathway, and is derived from Yarrowia lipolytica ATCC#20362. A six chip study using total RNA recovered from three separate cultures of Yarrowia lipolytica Y4184Ura+ strains and three separate cultures of Y4184 snf1 mutant strains in which YlSNF1 gene is deleted entirely. Each chip measures the expression level of 6708 genes from Yarrowia lipolytica ATCC#20362 with fourteen 24-mer probe pairs (PM/MM) per gene, with three-fold technical redundancy.
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