Project description:Pseudomonas fragi is the predominant bacterial species associated with spoiled aerobically stored chilled meat worldwide. It readily forms biofilms on meat under refrigerated temperature conditions used in meat industry. Biofilm growth leads to slime development on meat which becomes a major quality defect. In this research, RNA sequencing was carried out for the main stages of P. fragi strain 1793 grown on aerobically stored meat, kept at 10 °C. RNA was extracted at different stages of the biofilm cycle namely initiation, maturation and dispersal. The key objectives of this study was to investigate which genes are expressed at each of these stages as well as to understand the causes of P. fragi biofilm dipersal.
Project description:St. 14 embryos were incubated in 100mM of a Jnk inhibitor (SP600125)-0,1x MBS until stage 24. Embryonic epidermis was isolated and stored immediately in chilled (4°C) Trizol. We sought to uncover all the putative targets of Jnk expresssed in the embryonic epidermis whole embryonic skin was isolated and immediately stored in chilled trizol
Project description:This study investigated changes in gene expression of controlled environment chilled (4C) grape overwintering buds as they accumulated from 0 to 2000 chilling hours. Keywords: time course, chilling, endodormancy release, axillary bud, grape
