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Expression data from purified human long bone fracture callus Skeletal Stem Cells (SSCs) and Osteogenic Progenitor (hOPs) and Chondrogenic Progenitor (hCPs) cells

ABSTRACT: Expression data from purified human long bone fracture callus Skeletal Stem Cells (SSCs) and Osteogenic Progenitor (hOPs) and Chondrogenic Progenitor (hCPs) cells

PROVIDER: PRJNA872544 | ENA |

REPOSITORIES: ENA

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Expression data from purified human long bone fracture callus Skeletal Stem Cells (SSCs) and Osteogenic Progenitor (hOPs) and Chondrogenic Progenitor (hCPs) cells

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Expression data from purified one-week mouse fracture callus Skeletal Stem Cells (SSCs) and Bone-Cartilage-Stromal Progenitor cells (BCSPs)

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Fracture callus from young and aged mice

Project description:Skeletal stem/progenitor cells are critical for fracture repair by providing osteochondro precursors in the callus, which is impaired in aging. However, the molecular signatures of callus skeletan progenitor cells during aging is not known. We performed single-cell RNA sequencing on CD45-CD31-Ter119- skeletan progenitor cells isolated from young and aged mouse calluses.

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Gene expression in alpha SMA Cre-labeled cells in periosteum during early fracture callus formation

Project description:Fracture healing is a process that involves many cell populations. In this study we characterized gene expression in a subset of cells involved in fracture healing. αSMACreERT2 mice crossed with Ai9 reporter mice that express tdTomato fluorescent protein after Cre-mediated activation were used as an experimental model. αSMA-expressing cells were labeled by tamoxifen administration, then periosteal cells from the tibia were isolated two days later (controls), or tibial fractures were performed and periosteum/soft callus tissue was collected after 2 and 6 days. The tdTomato positive cell population was isolated by flow cytometry, and subjected to microarray analysis. Histology and cell surface marker analysis indicates that αSMACreERT2 labels a mainly mesenchymal population in the periosteum that expands after fracture, and contributes to both osteogenic and chondrogenic elements of the fracture callus. We were therefore able to examine gene expression in a defined population during the early stages of fracture healing. Total RNA was obtained from the tomato positive cells within the periosteal compartment of fractures from αSMACreERT2/Ai9 mice. Control animals were given 2 doses of tamoxifen, and periosteum was collected and labeled cells sorted (8-9 sex-matched mice per group). Fractures were performed after the second dose of tamoxifen, and tomato positive cells from periosteum/callus tissue were isolated 2 and 6 days after fracture (4-8 animals per sample pooled). 3 replicates for each sample are included.

2013-11-27 | E-GEOD-45156 | biostudies-arrayexpress

Gene expression in alpha SMA Cre-labeled cells in periosteum during early fracture callus formation

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miRNA expression data from callus in standard fracture healing models and from fibrous tissues in nonunion models in rats on post-fracture day 14

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