Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Long-term engraftment of engineered human plasma cells

ABSTRACT: Long-term engraftment of engineered human plasma cells

PROVIDER: PRJNA873089 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR21188830.fastq.gz	Fastqsanger.gz
	SRR21188831.fastq.gz	Fastqsanger.gz
	SRR21188832.fastq.gz	Fastqsanger.gz
	SRR21188833.fastq.gz	Fastqsanger.gz
	SRR21188834.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 64

Similar Datasets

Long-term engraftment of engineered human plasma cells

Project description:Due to their unique longevity and capacity to secrete high levels of protein, plasma B cells play have the potential to be used as a cell therapy for protein replacement. Here, we show that ex vivo engineered human plasma cells exhibited transcriptional features of long-lived plasma cells.

2022-09-15 | GSE211984 | GEO

Stromal Repair via Long-term Engraftment of Primary Bone Marrow Stroma Improves Hematopoietic Stem Cell Transplantation

Project description:Stromal Repair via Long-term Engraftment of Primary Bone Marrow Stroma Improves Hematopoietic Stem Cell Transplantation

2017-07-02 | GSE79091 | GEO

CITE-seq ex vivo engineered human plasma cells

2022-09-15 | GSE212138 | GEO

Human IL-6 fosters long-term engraftment of human patient derived myeloma cells in immunodeficient mice

Project description:Multiple myeloma is a largely incurable and life-threatening malignancy of antibody-secreting plasma cells. An effective and widely available animal model that recapitulates human myeloma and related plasma cell disorders is lacking. We show that busulfan-conditioned hIL-6 transgenic NSG mice (NSG+hIL6) reliably support the engraftment of malignant and pre-malignant human plasma cells including from patients diagnosed with pre- and post-relapse myeloma, plasma cell leukemia, and AL amyloidosis. Consistent with human disease, NSG+hIL6 mice engrafted with patient-derived myeloma cells developed blood M spikes, and a majority developed anemia, hypercalcemia, and/or bone lesions. Singe cell RNAseq analyses showed non-malignant and malignant cell engraftment, the latter expressing a wide array of mRNAs associated with myeloma cell survival and proliferation. Myeloma-engrafted mice given CAR T-cells targeting plasma cells or bortezomib experienced reduced tumor burden. Our results establish that NSG+hIL6 mice provide an effective patient derived xenograft model for study and preclinincal drug development for myeloma and related plasma cell disorders.

2024-10-14 | GSE246140 | GEO

Infection drives long-term meningeal engraftment by inflammatory monocytes that impair central nervous system immunity

Project description:Infection drives long-term meningeal engraftment by inflammatory monocytes that impair central nervous system immunity

| PRJNA428167 | ENA

High-throughput characterization of hematopoietic stem cell engraftment after intravenous and intracerebroventricular dosing

Project description:Hematopoietic stem/progenitor cell gene therapy (HSPC-GT) has shown clear neurological benefit in rare diseases, which is achieved through the engraftment of genetically modified microglia-like cells (MLCs) in the brain. Still, the engraftment dynamics and the nature of engineered MLCs, as well as their potential use in common neurogenerative diseases have remained largely unexplored. Here, we firstly comprehensively characterized how different routes of administration affect the biodistribution of genetically engineered MLCs and other HSPC-derivatives in mice. We then generated a high-resolution single cell transcriptional map of MLCs and discovered that they could clearly be distinguished from macrophages as well as from resident microglia by the expression of a specific gene signature which is reflective of their HSPC ontogeny and irrespective of their long-term engraftment history. Lastly, using murine models of Parkinson’s disease and frontotemporal dementia, we demonstrated that MLCs can deliver therapeutically relevant levels of protein to the brain, thereby opening avenues for the clinical translation of HSPC-GT to the treatment of major neurological diseases.

2022-05-31 | GSE201756 | GEO

Human IL-6 fosters long-term engraftment of human patient derived myeloma cells in immunodeficient mice

Project description:Human IL-6 fosters long-term engraftment of human patient derived myeloma cells in immunodeficient mice

| PRJNA1031637 | ENA

HMGA2 Promotes Long-Term Engraftment and Myelo-Erythroid Differentiation of Human Hematopoietic Stem and Progenitor Cells

Project description:HMGA2 Promotes Long-Term Engraftment and Myelo-Erythroid Differentiation of Human Hematopoietic Stem and Progenitor Cells

| PRJNA420689 | ENA

Infection drives long-term meningeal engraftment by inflammatory monocytes that impair central nervous system immunity [I]

Project description:Infection drives long-term meningeal engraftment by inflammatory monocytes that impair central nervous system immunity [I]

| PRJNA427333 | ENA

Infection drives long-term meningeal engraftment by inflammatory monocytes that impair central nervous system immunity [II]

Project description:Infection drives long-term meningeal engraftment by inflammatory monocytes that impair central nervous system immunity [II]