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Defining key transcriptional regulators of extravillous trophoblast differentiation [RNA-seq]

ABSTRACT: Defining key transcriptional regulators of extravillous trophoblast differentiation [RNA-seq]

PROVIDER: PRJNA874733 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21297012.fastq.gz	Fastqsanger.gz
	SRR21297013.fastq.gz	Fastqsanger.gz
	SRR21297014.fastq.gz	Fastqsanger.gz
	SRR21297015.fastq.gz	Fastqsanger.gz
	SRR21297016.fastq.gz	Fastqsanger.gz

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Defining key transcriptional regulators of extravillous trophoblast differentiation

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Project description:During early human pregnancy, extravillous trophoblasts (EVT) play a central role in placental anchorage and blood vessel remodeling. Despite the essential roles in pregnancy, key factors and mechanisms underlying EVT differentiation remain largely unknown. Here, we defined key transcription factors (TFs) by monitoring the dynamics of transcriptome and enhancer usage during EVT differentiation. We confirmed the requirements of the defined TFs and identified their action mechanisms.

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Project description:To better understand how DNA methylation influences placentation, DNA from first trimester primary trophoblast populations (side-population trophoblasts, cytotrophoblasts and extravillous trophoblasts) isolated using FACS underwent reduced representation bisulfite sequencing and were compared to publicly available data of blastocyst-derived and somatic cell populations.

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